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Contactless Trace Oxygen Measurements
Oxygen Sensor Spot SP-PSt8-YAU
These oxygen sensor spots are most versatile tools for precise oxygen monitoring in any transparent or semi-transparent vessel. The spots are attached to the inner container surface, and measurements are taken contactless from the outside – through the vessel wall. The SP-PSt8-YAU measure dissolved as well as gaseous O2. They have a measurement range from 0 – 10 % O2, with a detection limit of 0.007 % O2. The SP-PSt8-YAU can be autoclaved.
- Non-invasive measurements through the vessel wall
- No consumption of oxygen
- Signal independent of flow velocity
- Measures oxygen in liquids as well as in gas phase
Applications
Bioprocess Development: Oxygen Monitoring in Shake Flasks
O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.
Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials
Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.
Technical
| Specifications | Gaseous & Dissolved O2 | Dissolved O2 |
|---|---|---|
| *after two-point calibration as described in the manual | ||
| Measurement range | 0 – 10 % O2 0 – 100 hPa | 0 – 4.5 mg/L 0 – 140 µmol/L |
| Limit of detection | 0.007 % oxygen | 3 ppb |
| Resolution | ± 0.002 % O2 at 0.008 % O2 | ± 0.7 ppb at 3 ppb |
| Accuracy at + 20 °C* | ± 3 ppb or ± 3 % rel. | |
| Measurement temperature range | from 0 to + 50 °C | |
| Response time (t90) | < 6 sec. | < 40 sec. |
| Properties | ||
| Compatibility | Aqueous solutions, ethanol, methanol | |
| No cross-sensitivity | pH 1 – 14 | |
| Cross-sensitivity | Organic solvents, such as acetone, toluene, chloroform or methylene chloride | |
| Sterilization procedure | Steam sterilization Ethylene oxide (EtO) | |
| Cleaning procedure | Cleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F) | |
| Calibration | Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and a second calibration value optimally between 2 % and 4 % oxygen | |
| Storage stability | 60 months provided the sensor material is stored in the dark at room temperature | |
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Resources
FAQs
Can I sterilize fiber optic sensors?
Do I have to mount the sensors in the dark because it says "Protect from light" on the packing?
Do the sensors work in turbid solutions?
Does each oxygen sensor spot need a separate calibration, or are the calibration values valid for the complete sensor spot batch?
How can I convert an oxygen value into a different oxygen unit?
How do I import data into excel from a text file containing measurement data?
How does an oxygen sensor work?
How does salinity affect the oxygen measurement?
How does temperature affect the oxygen measurement?
How many autoclaving cycles can an optical oxygen sensor go through without affecting the sensor performance?
In which vessel can I integrate a sensor spot?
Is the glue or the sensor spot biocompatible - which tests were done? Are there any studies on leachables and extractables?
Is there a standard recommended distance to place the polymer optical fiber (POF) from the sensor spot?
The Stern-Volmer-equation
What does -YAU, -NAU or -SA stand for in the oxygen sensor spot names and what is the difference between those sensor types?
What factors will affect the oxygen reading?
What is the time of delivery?
Where do I need sensor spot sizes different from the common diameter of 5 mm (approx. 0.2 inch)?
Which glue can I use to integrate optical sensor spots?
Which side of the oxygen sensor spot should face the medium?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Manuals
Brochures
Software
PreSens Oxygen Calculator version 3.1.1 - Windows 7/8/10