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Contactless Trace Oxygen Measurements

Oxygen Sensor Spot SP-PSt8-YAU

These oxygen sensor spots are most versatile tools for precise oxygen monitoring in any transparent or semi-transparent vessel. The spots are attached to the inner container surface, and measurements are taken contactless from the outside – through the vessel wall. The SP-PSt8-YAU measure dissolved as well as gaseous O2. They have a measurement range from 0 – 10 % O2, with a detection limit of 0.007 % O2. The SP-PSt8-YAU can be autoclaved.

  • Non-invasive measurements through the vessel wall
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measures oxygen in liquids as well as in gas phase
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Applications

Bioprocess Development: Oxygen Monitoring in Shake Flasks

O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.


Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Technical

Specifications Gaseous & Dissolved O2 Dissolved O2
*after two-point calibration as described in the manual
Measurement range 0 – 10 % O2
0 – 100 hPa

0 – 4.5 mg/L

0 – 140 µmol/L
Limit of detection 0.007 % oxygen 3 ppb
Resolution

± 0.002 % O2 at 0.008 % O2
± 0.06 % O2 at 2.5 % O2

± 0.7 ppb at 3 ppb
± 2.5 ppb at 1000 ppb

Accuracy at + 20 °C* ± 3 ppb or ± 3 % rel.
Measurement temperature range from 0 to + 50 °C
Response time (t90) < 6 sec. < 40 sec.
Properties    
Compatibility Aqueous solutions, ethanol, methanol
 
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivity

Organic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas

Sterilization procedure Steam sterilization
Ethylene oxide (EtO)
Cleaning procedure

Cleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
3 % H2O2, ethanol, soap solution

Calibration Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and a second calibration value optimally between 2 % and 4 % oxygen
Storage stability 60 months provided the sensor material is stored in the dark at room temperature

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