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High Resolution Profiling of Oxygen Traces

Profiling Oxygen Microsensor PM-PSt8

The PM-PSt8 is specifically designed for profiling applications. It is the most robust microsensor version PreSens offers – with a firmer fiber construction and a splash-proof metal housing. Therefore, it is ideally suited for profiling applications or whenever minimal invasive trace oxygen measurements need to be performed in semi-solid samples, like e. g. sediments, microbial mats or biofilms. The PM-PSt8 is compatible with the Microx 4 trace and OXY-1 ST trace oxygen meters. It has a measurement range of 0 % – 10 % oxygen, with a detection limit of 0.007 % oxygen.

  • High spatial resolution (< 50 μm)
  • High temporal resolution (t90 < 3 sec.)
  • Penetration probe for insertion into semi-solids
  • Mechanical interlock for precise vertical localization inside the sample
  • Splash-proof metal housing
  • No electrical interference due to optical measurements

Applications

Microprofiling in Biological & Environmental Research

The different types of oxygen and pH microsensors allow e. g. measurements in smallest sample volumes or inside tissue. The micromanipulators should be applied whenever it is necessary to insert the microsensor safely into semi-solid samples and when exact localization and stabilization of the microsensor tip within the sample is required. Using the safe-insert function the microsensor tip can be securely inserted and localized at the exact position where you want to conduct your measurements.


Microsensor Measurements in Medical & Life Science Research

PreSens microsensors are ideal tools for medical and life science research, as they allow for precise on the spot measurement and microprofiling inside tissue constructs. The Manual Micromanipulator is the indispensable equipment in these applications for exact localization of the microsensor inside the sample and microprofiling in step sizes down to 10 μm. PreSens needle-type microsensors are used in many tissue engineering applications.


Microprofiling of Sediments & Biofilms

Together with the specially designed PreSens Profiling Microsensors (PM) the Automated Micromanipulator is the ideal tool for oxygen measurements in sediment and biofilm applications. With a free choice of step zones, travel velocities and wait times different layers inside the sample can be monitored and assessed in step sizes down to 10 μm. The software visualizes the online measurements, so you can follow gradients and identify boundaries immediately while the sensor is automatically moved inside the sample.


Technical

Specifications*Gaseous & Dissolved O2Dissolved O2
*data for microsensors TF = flat-broken sensor tip with 230 μm diameter
**after two-point calibration as described in the manual
Measurement range0 – 10 % O2
0 – 100 hPa

0 – 4.5 mg/L

0 – 140 µmol/L
Limit of detection0.007 % oxygen3 ppb
Resolution

± 0.002 % O2 at 0.008 % O2
± 0.06 % O2 at 2.5 % O2

± 0.7 ppb at 3 ppb
± 2.5 ppb at 1000 ppb

Accuracy at + 20 °C**± 3 ppb or ± 3 % rel.
Measurement temperature rangefrom 0 to + 50 °C
Response time (t90)< 3 sec. (gas)< 10 sec. (liquid)
Properties
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivity

Organic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas

Sterilization procedureEthylene oxide (EtO)
Cleaning procedure

3 % H2O2
Ethanol
Soap solution

CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and a second calibration value optimally between 2 % and 4 % oxygen
Storage stability60 months provided the sensor material is stored in the dark at room temperature

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