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Oxygen Monitoring in 96-well Microtiterplate
OxoPlate OP96U
The OP96U is a round bottom 96-well multiplate with an oxygen sensor integrated on the bottom of each well. The plate is made of polystyrene. The sensors can be read out in parallel from the bottom side using a commercially available fluorescence reader.
- Ready-to-use
- User-defined batch calibration
- For use with conventional microplate fluorescence readers
- High throughput screening
Applications
Enzyme Kinetics Measurement
The OxoPlate OP96U can be used to determine enzyme activities. In this example kinetics of glucose oxidase (GOD) with different activities (0.05, 0.02, 0.01 and 0.05 U/mL) is shown. The OxoPlate filled with glucose solution and catalase (to avoid destruction of glucose oxidase by formed hydrogen peroxide) was incubated in the reader before the enzyme was added. Praffine oil was put into each well to reduce oxygen ingress. The oxygen measurement results show clearly the different dynamic equilibria of oxygen consumption and oxygen ingress depending on the added enzymes activity.
S. Arain, 2006, Microrespirometry with Sensor-Equipped Microtiterplates, University of Regensburg, Regensburg, Germany
Technical
| Specifications* | |
|---|---|
| * in H2O dest. or oxygen-free water | |
| Measurement range | 0 - 30 % O2 |
| Resolution | ± 0.2 % O2 at 0.21 % O2 ± 0.6 % O2 at 20.9 % O2 |
| Accuracy | ± 0.4 % O2 at 0.21 % O2 ± 1.0 % O2 at 20.9 % O2 |
| Drift | < 0.04 % O2 per hour (sampling interval 1 min.) |
| Measurement Temperature Range | from + 15 °C to + 45 °C |
| Response time (t90) | at + 25 °C: < 30 sec. |
| Properties | |
| Compatibility | Aqueous solutions, ethanol (max . 10 % v/v), methanol (max. 10 % v/v), pH 2 – 10, 20 % (v/v) DSMO, DMF, acetone, THF, or dioxane in solution. (Calibration constants have to be determined using the respective solvent instead of water.) |
| Cross sensitivity | Gaseous sulfur dioxide (SO2) and gaseous chlorine (Cl2); Changes in ionic strength |
| Calibration | Needs to be calibrated once per batch Disposables are delivered beta-irradiated |
| Plate type | 96-well round bottom plates (Greiner Microlon® 600) |
| Maximum filling volume | 300 µL |
| Indicator filters | 540 nm / 650 nm |
| Reference filters | 540 nm / 590 nm |
Resources
FAQs
Are the fluorescence intensities I get from the SensorPlate with my reader sufficient for a good measurement?
How do I know that my reader gives precise results with the SensorPlates?
How often do I have to calibrate the SensorPlates with my reader?
I formerly used the Oxygen BioSensor (OBS) from BD. Can I use the OxoPlate as a substitute?
I try to investigate oxygen consumption in my cell culture. However, the oxygen level stays at 100 % O2. Is there something wrong with the SDR measurements, and what can I do?
Is my microplate reader compatible to the SensorPlates?
The filters of my reader do not exactly match the combination in the SensorPlates' technical data. Nevertheless, can I use them?
The intensities I get with the SensorPlates are very low for my reader. How can I enhance them?
What is the time of delivery?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Why are the fluorescence intensities and ratios I get with my reader different from the ones in the SensorPlate manual?