Oxygen Monitoring in Perfusion Systems with Small Volumes

Oxygen Microsensor Integrated in a Metal Flow-Through Cell FTCM-PSt1

A microsensor is integrated in a stainless steel tee with connectors for 1/16” steel tubing. The flow-through cell’s inner volume of 2.1 µL is extraordinarily small. It is suited for all applications where only small volumes are available. The tapered sensor type ensures real-time measurement with its fast response time of t90 < 1 sec. in gas and < 5 sec. in liquids.

  • Perfusion monitoring in smallest volumes
  • High temporal resolution (< 5 sec.)
  • No consumption of oxygen
  • Measures in liquids as well as in the gas phase
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Specifications Gaseous & Dissolved O2 Dissolved O2
*after two-point calibration as described in the manual
**TS: tapered sensor tip with a diameter < 50 μm and no optical isolation
***TF: flat-broken sensor tip with a diameter of 140 μm
Measurement range

0 – 50 % O2

0 – 500 hPa

0 – 22.5 mg/L

0 – 700 µmol/L
Limit of detection 0.05 % oxygen 20 ppb

± 0.01 % O2 at 0.21 % O2
± 0.09 % O2 at 20.9 % O2
±0.1 hPa at 2 hPa

± 1 hPa at 207 hPa

± 0.005 mg/L at 0.09 mg/L
± 0.04 mg/L at 9.06 mg/L
± 0.14 µmol/L at 2.83 µmol/L

± 1.3 µmol/L at 283 µmol/L
Accuracy* ± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2
Drift < 0.1 % O2 within 30 days (sampling interval of 1 min.)
Measurement temperature range from 0 to + 50 °C
Response time TS** (t90) < 1 sec. (gas) < 2 sec. (liquid)
Response time TF*** (t90) < 15 sec. (gas) < 30 sec. (liquid)
Compatibility Aqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivity Organic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure

Ethylene oxide (EtO)

Cleaning procedure 3 % H2O2
Soap solution
Calibration Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability 60 months provided the sensor material is stored in the dark at room temperature

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