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SP-PSt6-NAU optical O2 sensor for trace range measurements

Trace Oxygen Measurements under Real Conditions

Oxygen Sensor Spot SP-PSt6-NAU

Sensor spots are the most versatile version of non-invasive optical oxygen sensors. They can be attached to the inner surface of any transparent glass or plastic vessel like e. g. used for packaging. Oxygen is measured contactless through the transparent vessel wall. The SP-PSt6-NAU has a measurement range of 0 – 5 % oxygen, dissolved or in the gas phase. The oxygen sensitive coating is immobilized on a 125 µm flexible transparent polyester foil, which does not stand autoclaving.

  • Non-invasive measurements through the vessel wall
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measures oxygen in liquids as well as in gas phase
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Applications

Bioprocess Development: Oxygen Monitoring in Shake Flasks

O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.


Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Technical

SpecificationsGaseous & Dissolved O2Dissolved O2
*after two-point calibration as described in the manual
Measurement range

0 – 5  % O2

0 – 41.4 hPa

0 – 2 mg/L

0 – 56.9 µmol/L
Limit of detection0.002 % oxygen

1 ppb

Resolution

± 0.0007 % O2 at 0.002 % O2
± 0.0015 % O2 at 0.2 % O2
± 0.007 hPa at 0.023 hPa

± 0.015 hPa at 2.0 hPa

± 0.0003 mg/L at 0.001 mg/L
± 0.0006 mg/L at 0.09 mg/L
± 0.010 µmol/L at  0.03µmol/L

± 0.020 µmol/L at 2.8 µmol/L
Accuracy*± 1 ppb or ± 3 % of the respective concentration; whichever is higher
Drift at 0 % oxygen< 2 ppb within 30 days (sampling interval of 1 min.)
Measurement temperature rangefrom 0 to + 50 °C
Response time (t90)< 6 sec.< 40 sec.
Properties  
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivityOrganic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure

Ethylene oxide (EtO)

Cleaning procedure

Cleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 % – 5 %

CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and a second calibration value optimally between 1 % and 2 % oxygen
Storage stability60 months provided the sensor material is stored in the dark

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Resources

Publications

Dissolved Oxygen Determination Determining the Impact of Shoreline Retrogressive Thermokarst Slumping to Tundra Lakes Development of a New, Highly Sensitive Sensor for Permeation Measurement Anaerobic sulfur oxidation underlies adaptation of a chemosynthetic symbiont to oxic-anoxic interfaces From the bottom of the sea to the display case: A study into the long-term preservation of archaeological maritime silk textiles in controlled atmosphere Acetaldehyde metabolism in industrial strains of Saccharomyces cerevisiae inhibited by SO2 and cooling during alcoholic fermentation Manipulating the strength of organism-environment feedback increases nonlinearity and apparent hysteresis of ecosystem response to environmental change Hydrogen utilization by Methylocystis sp. strain SC2 expands the known metabolic versatility of type IIa methanotrophs Regulation of Primary Metabolism in Response to Low Oxygen Availability as Revealed by Carbon and Nitrogen Isotope Redistribution Gas permeability of polyurethane films for fresh produce packaging: Response of O2 permeability to temperature and relative humidity Comparison of test methods for oxygen permeability: Optical method versus carrier gas method Competition between Oxygen and Nitrate Respirations in Continous Culture of Pseudomonas aeruginosa Performing Aerobic Denitrification Kinetics of chlorobenzene biodegradation under reduced oxygen levels Complementary Methods for the Determination of Dissolved Oxygen Content in Perfluorocarbon Emissions and Other Solutions Temperature-dependent Oxygen Permeation through PET/MXD6-Barrier Blend Bottles with and without Oxygen Absorber Aquatic Respiration Rate Measurements at Low Oxygen Concentrations Influence of plasticizers on the mechanical and barrier properties of cast biopolymer films

Software

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