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Planar Trace Oxygen-Sensitive Spot

Oxygen Sensor Spot SP-PSt6-YAU

These sensor spots are the most versatile version of optical trace oxygen sensors. They can be attached to the inner surface of any transparent glass or plastic vessel like e. g. used for packaging. Oxygen is measured contactless and non-destructively through the transparent vessel wall. The SP-PSt6-YAU has a measurement range of 0 – 5 % oxygen in dissolved or gaseous phase. The oxygen sensitive coating is immobilized on a 1 mm glass support, which can be autoclaved.

  • Non-invasive measurements in a range of 0 - 5 % oxygen
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measures oxygen in liquids as well as in gas phase
  • Autoclavable
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Applications

Bioprocess Development: Oxygen Monitoring in Shake Flasks

O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.


Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Technical

Specifications Gaseous & Dissolved O2 Dissolved O2
*after two-point calibration as described in the manual
Measurement range

0 – 5  % O2

0 – 41.4 hPa

0 – 2 mg/L

0 – 56.9 µmol/L
Limit of detection 0.002 % oxygen

1 ppb

Resolution

± 0.0007 % O2 at 0.002 % O2
± 0.0015 % O2 at 0.2 % O2
± 0.007 hPa at 0.023 hPa

± 0.015 hPa at 2.0 hPa

± 0.0003 mg/L at 0.001 mg/L
± 0.0006 mg/L at 0.09 mg/L
± 0.010 µmol/L at  0.03µmol/L

± 0.020 µmol/L at 2.8 µmol/L
Accuracy* ± 1 ppb or ± 3 % of the respective concentration; whichever is higher
Drift at 0 % oxygen < 2 ppb within 30 days (sampling interval of 1 min.)
Measurement temperature range from 0 to + 50 °C
Response time (t90) < 6 sec. < 40 sec.
Properties    
Compatibility Aqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivity Organic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure

Steam sterilization
Ethylene oxide (EtO)

Cleaning procedure

Cleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 % – 5 %

Calibration Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and a second calibration value optimally between 1 % and 2 % oxygen
Storage stability 60 months provided the sensor material is stored in the dark

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Resources

FAQs

Can I sterilize fiber optic sensors? Do I have to mount the sensors in the dark because it says "Protect from light" on the packing? Do the sensors work in turbid solutions? Does each oxygen sensor spot need a separate calibration, or are the calibration values valid for the complete sensor spot batch? How can I convert an oxygen value into a different oxygen unit? How do I import data into excel from a text file containing measurement data? How does an oxygen sensor work? How does salinity affect the oxygen measurement? How does temperature affect the oxygen measurement? How many autoclaving cycles can an optical oxygen sensor go through without affecting the sensor performance? In which vessel can I integrate a sensor spot? Is the glue or the sensor spot biocompatible - which tests were done? Are there any studies on leachables and extractables? Is there a standard recommended distance to place the polymer optical fiber (POF) from the sensor spot? The Stern-Volmer-equation What are the response times for the oxygen sensors based on the 2 mm fiber like non-invasive oxygen sensors and oxygen probes? What does -YAU, -NAU or -SA stand for in the oxygen sensor spot names and what is the difference between those sensor types? What factors will affect the oxygen reading? What is the time of delivery? Where do I need sensor spot sizes different from the common diameter of 5 mm (approx. 0.2 inch)? Which glue can I use to integrate optical sensor spots? Which side of the oxygen sensor spot should face the medium? Which substances can interfere with the optical O2, pH and CO2 measurements?

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