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Contactless Oxygen Measurements
Oxygen Sensor Spot SP-PSt7-YAU
These oxygen sensor spots are most versatile tools for precise oxygen monitoring in any transparent or semi-transparent vessel. Measurements are then taken contactless from the outside – through the vessel wall. The SP-PSt7-YAU measure dissolved as well as gaseous O2. They have a measurement range from 0 – 100 % O2, with a detection limit of 0.03 % O2. The SP-PSt7-YAU can be autoclaved.
- Non-invasive measurements through the vessel wall
- No consumption of oxygen
- Signal independent of flow velocity
- Measures oxygen in liquids as well as in gas phase
Applications
Bioprocess Development: Oxygen Monitoring in Shake Flasks
O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.
Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials
Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.
Technical
Specifications | Gaseous & Dissolved O2 | Dissolved O2 |
---|---|---|
*after two-point calibration as described in the manual | ||
Measurement range | 0 – 100 % O2 0 – 1000 hPa | 0 – 45 mg/L 0 – 1400 µmol/L |
Limit of detection | 0.03 % oxygen | 15 ppb |
Resolution | ± 0.01 % O2 at 1 % O2 | ± 0.005 mg/L at 0.4mg/L ± 0.025 mg/L at 9.06 mg/L |
Accuracy at + 20 °C* | ± 0.05 % O2 or ± 3 % rel. | |
Measurement temperature range | from 0 to + 50 °C | |
Response time (t90) | < 6 sec. | < 40 sec. |
Properties | ||
Compatibility | Aqueous solutions, ethanol, methanol | |
No cross-sensitivity | pH 1 – 14 | |
Cross-sensitivity | Organic solvents, such as acetone, toluene, chloroform or methylene chloride | |
Sterilization procedure | Steam sterilization Ethylene oxide (EtO) | |
Cleaning procedure | Cleaning in Place (CIP, 2 % NaOH, + 80 °C, + 176 °F) | |
Calibration | Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment | |
Storage stability | 60 months provided the sensor material is stored in the dark at room temperature |
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Software
PreSens Oxygen Calculator version 3.1.1 - Windows 7/8/10