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Modular Control and Evaluation Software for VisiSens TD

VisiSens™ ScientifiCal Software

VisiSens TD includes a modular control and evaluation software. One can choose between different operation modes from single- to multi-analyte modes. Images can be recorded as snapshots or automatic time series. Furthermore, the software offers different evaluation functions for image analyses.

  • Modular control and evaluation software
  • Single- to multi-analyte modes
  • Snapshot or time series recordings
  • Image evaluation functions

Applications

Visualizing Analytes over Large Sample Surfaces

The VisiSens TD images large areas and gathers information on spatial and temporal analyte changes, e. g. in large scale pH and CO2 imaging of root-soil interactions within rhizotrons or when analyzing biogeochemical processes in sediments. It is also well suited for application in sensitive processes, e. g. metabolic activity of living samples or tissue engineering, where O2 gradients over a cross section of a 3D graft can be monitored.


Multi-Sample and Multi-Sensor Read-Out

Large field of views enable analyte value recording in multiple samples simultaneously with free choice of measurement geometry. Even different sensor ranges can be combined. The fluorescent sensor foils can be mounted at the bottom of transparent vessels, plates or vials containing different samples and placed next to each other, e. g. in a small rack. One image taken from the bottom contains the information on analyte concentrations in a group of samples.


Three Different Analytes with one Device

The modular set-up is applicable for imaging oxygen, pH, and CO2 sensor foils with one system and in one field of view. It visualizes spatial and temporal changes of analyte concentrations. The combined information on distributions of several analytes can give valuable insight in metabolic activity or chemical processes inside samples, e. g. when investigating complex processes in cell culture, or tissues, in root systems, or when phenotyping crop plants.


Microscopic Analyte Imaging - TD MIC Configuration

VisiSens TD can be equipped with microscope optics for measuring analyte gradients with very high spatial resolution. The VisiSens TD MIC can be used e.g. to monitor spatial and temporal changes of O2 of cross-sections of multicellular tumor spheroids. The system is compact and fits into an incubator to monitor analyte changes under cell culture conditions over days.


Technical

  Minimum System Requirements Suggested Configuration
Operating system

Microsoft® Windows® 7 (32 or 64 Bit) or higher

Microsoft® Windows® 10 (64 Bit)
Processor 2.4 GHz Pentium IV or adequate AMD Athlon Processor Intel ‘i ‘ series or adequate AMD Processor (or higher)
RAM 2 GB 8 GB or more
Memory capacity for graphic board 256 MB 1 GB or more
Hard disk 1 GB free memory 2 TB or more free memory
Ethernet 10/100, or Gigabit speed Ethernet adapters Gigabit speed Ethernet adapters
If not on-board:
For desktop system: Use a PCI Express bus Ethernet adapter.
For laptops: Use an expansion slot via an ExpressCard.
Screen resolution

1920 x 1080 (16 : 9) or
1366 x 768 (16 : 9) or
1280 x 800 ( 16 : 10) or
1280 x 1024 (5 : 4)

1680 x 1050 or higher

(16:9 or 16:10)

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Resources

Publications

In situ Remediation of Contaminated Groundwater Monitoring Spatio-Temporal Oxygen Consumption Within Live 3D Cancer Spheroids Testing Plant Seed Viability by Respiration Measurements Spatio-temporal O2 Gradients in the Microenvironment of an Outgrowing Cell Patch Oxygen Consumption Rates of Adherent MDCK II Monolayer Cells Imaging of pH and pO2 on Irradiated Fibroblasts and Oral Squamous Carcinoma Cells O2-sensitive microcavity arrays: A new platform for oxygen measurements in 3D cell cultures Physiological oxygen measurements in vitro – Schrödinger´s cat in 3D cell biology O2-sensitive Mikrokavitätenarrays: 3D-Zellkultursystem mit Sensorfunktion Plant-Mediated Rhizosphere Oxygenation in the Native Invasive Salt Marsh Grass Elymus athericus A New Non-invasive Technique for Measuring 3D-Oxygen Gradients in Wells During Mammalian Cell Culture In situ observation of pH change during water splitting in neutral pH conditions: impact of natural convection driven by buoyancy effects Impact of Bed Form Celerity on Oxygen Dynamics in the Hyporheic Zone Scalable Microfluidic Platform for Flexible Configuration of and Experiments with Microtissue Multiorgan Models Iron Lung: How Rice Roots Induce Iron Redox Changes in the Rhizosphere and Create Niches for Microaerophilic Fe(II)-Oxidizing Bacteria Elevated micro-topography boosts growth rates in Salicornia procumbens by amplifying a tidally-driven oxygen pump: implications for natural recruitment and restoration Tomato plants rather than fertilizers drive microbial community structure in horticultural growing media Imaging of pH and pO2 gives insight in molecular processes of irradiated cells Experimental study on the oxygenation efficiency of nano-bubble modified mineral particles at the sediment-water interface in lakes Successful control of phosphorus release from sediments using oxygen nano-bubble-modified minerals Dynamics of oxygen and carbon dioxide in rhizospheres of Lobelia dortmanna - a planar optode study of belowground gas exchange between plants and sediment Plant-Sediment Interactions in Salt Marshes - An Optode Imaging Study of O2, pH, and CO2 Gradients in the Rhizosphere
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