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Modular O2, pH and CO2 Mapping System
VisiSens TD
VisiSens TD enables simultaneous 2D read-out of optical O2, pH and CO2 sensor foils within one set-up. Planar sensors for one or more analytes are placed on the sample area or in different cavities and the fluorescent sensor signals are read out pixel by pixel with a camera. Fields of view from mm2 up to 30 cm x 25 cm are possible. VisiSens TD gives an overview over your sample area and allows to freely choose the region of interest for investigation of spatial and temporal gradients or simultaneous read-out of multiple sensor signals. The modular system can be customized according to the users’ requirements.
- 3 analytes – 1 system
- Multiple sensor types combinable in one field of view
- Variable sensor and measurement geometry
- 12-bit detector
- Adaptable field of view, microscopic, 4 x 4 cm2 or up to 30 x 25 cm2
- Time-lapse slide shows of recordings
Applications
Visualizing Analytes over Large Sample Surfaces
The VisiSens TD images large areas and gathers information on spatial and temporal analyte changes, e. g. in large scale pH and CO2 imaging of root-soil interactions within rhizotrons or when analyzing biogeochemical processes in sediments. It is also well suited for application in sensitive processes, e. g. metabolic activity of living samples or tissue engineering, where O2 gradients over a cross section of a 3D graft can be monitored.
Multi-Sample and Multi-Sensor Read-Out
Large field of views enable analyte value recording in multiple samples simultaneously with free choice of measurement geometry. Even different sensor ranges can be combined. The fluorescent sensor foils can be mounted at the bottom of transparent vessels, plates or vials containing different samples and placed next to each other, e. g. in a small rack. One image taken from the bottom contains the information on analyte concentrations in a group of samples.
Three Different Analytes with one Device
The modular set-up is applicable for imaging oxygen, pH, and CO2 sensor foils with one system and in one field of view. It visualizes spatial and temporal changes of analyte concentrations. The combined information on distributions of several analytes can give valuable insight in metabolic activity or chemical processes inside samples, e. g. when investigating complex processes in cell culture, or tissues, in root systems, or when phenotyping crop plants.
Microscopic Analyte Imaging - TD MIC Configuration
VisiSens TD can be equipped with microscope optics for measuring analyte gradients with very high spatial resolution. The VisiSens TD MIC can be used e.g. to monitor spatial and temporal changes of O2 of cross-sections of multicellular tumor spheroids. The system is compact and fits into an incubator to monitor analyte changes under cell culture conditions over days.
Technical
| Oxygen (blue) | pH (red) | CO2 (green) | |||
|---|---|---|---|---|---|
| Specifications | SF-RPSu4 | SF-LV1R | SF-HP5R | SF-CD2R* | SF-CD1R |
| Measurement range | 0 - 100 % air saturation (0 - 20.9 % O2) | pH 2.5 - 4.5 | pH 5.5 - 7.5 | 0 - 1 % pCO2 at atmospheric pressure (1013.15 hPa) | 1 - 25 % pCO2 at atmospheric pressure (1013.15 hPa) |
| Response time (t90)** | Gas phase: < 8 sec. Dissolved: < 30 sec. | < 30 sec. | < 30 sec. | < 3 min. | < 3 min. |
| Limit of detection*** | 0.03 % air saturation | ||||
| Precision (temporal)**** | ± 0.02 % air saturation at 0 % air saturation ± 0.1 % air saturation at 100 % air saturation | ± 0.01 pH at pH = 4 | ± 0.01 pH at pH = 7 | ± 0.02 % CO2 at 0.15 % CO2 ± 0.02 % CO2 at 0.8 % CO2 | ± 0.02 % CO2 at 2% CO2 ± 0.1 % CO2 at 25% CO2 |
| Precision (spatial)***** | ± 1.5 % air saturation at 0% air saturation ± 3 % air saturation at 100 % air saturation | ± 0.1 pH at pH = 4 | ± 0.1 pH at pH = 7 | ± 0.08 % CO2 at 0.15 % CO2 ± 0.08 % CO2 at 0.8 % CO2 | ± 0.2 % CO2 at 2 % CO2 ± 1.2 % CO2 at 25 % CO2 |
| Properties | |||||
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10 | Aqueous solutions, pH 2 - 9 | Aqueous solutions, pH 4 - 9 | ||
| General sensor temperature working range | from + 5 °C to + 45 °C | ||||
| System Components | Ratiometric Imaging | ||
|---|---|---|---|
* Prototype component; please contact our service team! | |||
| Hardware | O2 | pH | CO2 |
| VisiSens TD Basic System* | x | x | x |
| Big Area Imaging kit* | x | x | x |
| TD MIC Configuration* | x | ||
| Software | |||
| VisiSens ScientifiCal* | |||
| Imaging Modality O2* | x | ||
| Imaging Modality pH* | x | ||
| Imaging Modality CO2* | x | ||
| Mixed dual or triple modes* | x | x | x |
| Sensor | |||
| SF-RPSu4 | x | ||
| SF-HP5R | x | ||
| SF-LV1R | x | ||
| SF-CD1R | x | ||
| SF-CD2R* | x | ||
| Accessories (optional) | |||
| CaliPlate - pH Calibration Helper Plate | |||
| Mounting Rack* | |||
| Sensor Plate Tubus Adapter* | |||
| Resolution Test Chart* | |||
| Plates with Integrated Sensor Foils* | |||
Related products
Resources
Publications
O2-sensitive Microcavity Arrays for 3D Cell Culture Monitoring
In situ Remediation of Contaminated Groundwater
Monitoring Spatio-Temporal Oxygen Consumption Within Live 3D Cancer Spheroids
Testing Plant Seed Viability by Respiration Measurements
Spatio-temporal O2 Gradients in the Microenvironment of an Outgrowing Cell Patch
Oxygen Consumption Rates of Adherent MDCK II Monolayer Cells
Imaging of pH and pO2 on Irradiated Fibroblasts and Oral Squamous Carcinoma Cells
Quantification of oxygen consumption in head and neck cancer using fluorescent sensor foil technology
O2-sensitive microcavity arrays: A new platform for oxygen measurements in 3D cell cultures
Physiological oxygen measurements in vitro – Schrödinger´s cat in 3D cell biology
O2-sensitive Mikrokavitätenarrays: 3D-Zellkultursystem mit Sensorfunktion
Plant-Mediated Rhizosphere Oxygenation in the Native Invasive Salt Marsh Grass Elymus athericus
A New Non-invasive Technique for Measuring 3D-Oxygen Gradients in Wells During Mammalian Cell Culture
In situ observation of pH change during water splitting in neutral pH conditions: impact of natural convection driven by buoyancy effects
Impact of Bed Form Celerity on Oxygen Dynamics in the Hyporheic Zone
Scalable Microfluidic Platform for Flexible Configuration of and Experiments with Microtissue Multiorgan Models
Iron Lung: How Rice Roots Induce Iron Redox Changes in the Rhizosphere and Create Niches for Microaerophilic Fe(II)-Oxidizing Bacteria
Elevated micro-topography boosts growth rates in Salicornia procumbens by amplifying a tidally-driven oxygen pump: implications for natural recruitment and restoration
Tomato plants rather than fertilizers drive microbial community structure in horticultural growing media
Imaging of pH and pO2 gives insight in molecular processes of irradiated cells
Experimental study on the oxygenation efficiency of nano-bubble modified mineral particles at the sediment-water interface in lakes
Successful control of phosphorus release from sediments using oxygen nano-bubble-modified minerals
Dynamics of oxygen and carbon dioxide in rhizospheres of Lobelia dortmanna - a planar optode study of belowground gas exchange between plants and sediment
Plant-Sediment Interactions in Salt Marshes - An Optode Imaging Study of O2, pH, and CO2 Gradients in the Rhizosphere
Cross-Reactive Metal Ion Sensor Array in a Micro Titer Plate Format
Ultrasonic welding of chemical optical sensors supporting O2, pH and CO2 imaging in microfluidic systems