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High Resolution Oxygen Profiling in Semi-Solid Samples
Profiling Oxygen Microsensor PM-PSt7
The PM-PSt7 is one of the most robust oxygen microsensor PreSens offers – with a firmer fiber construction and a splash-proof metal housing. It is specifically designed for profiling applications and should be used whenever minimal invasive measurements need to be performed in semi-solid samples, e. g. in sediments, microbial mats or biofilms. The PM-PSt7 is compatible with the Microx 4 and OXY-1 / -4 ST series oxygen meters and has a measurement range of 0 – 100 % oxygen, with a detection limit of 0.03 % oxygen. Together with the Automated Micromanipulator and the software PreSens Profiling Studio boundary layers and gradients can be followed online, while the sensor is automatically moved inside the sample.
- High spatial resolution (< 50 μm)
- High temporal resolution (t90 < 3 sec.)
- Penetration probe for insertion into semi-solids
- Mechanical interlock for precise vertical localization inside the sample
- Splash-proof metal housing
- No electrical interference due to optical measurements
Applications
Microprofiling in Biological & Environmental Research
The different types of oxygen and pH microsensors allow e. g. measurements in smallest sample volumes or inside tissue. The micromanipulators should be applied whenever it is necessary to insert the microsensor safely into semi-solid samples and when exact localization and stabilization of the microsensor tip within the sample is required. Using the safe-insert function the microsensor tip can be securely inserted and localized at the exact position where you want to conduct your measurements.
Microsensor Measurements in Medical & Life Science Research
PreSens microsensors are ideal tools for medical and life science research, as they allow for precise on the spot measurement and microprofiling inside tissue constructs. The Manual Micromanipulator is the indispensable equipment in these applications for exact localization of the microsensor inside the sample and microprofiling in step sizes down to 10 μm. PreSens needle-type microsensors are used in many tissue engineering applications.
Microprofiling of Sediments & Biofilms
Together with the specially designed PreSens Profiling Microsensors (PM) the Automated Micromanipulator is the ideal tool for oxygen measurements in sediment and biofilm applications. With a free choice of step zones, travel velocities and wait times different layers inside the sample can be monitored and assessed in step sizes down to 10 μm. The software visualizes the online measurements, so you can follow gradients and identify boundaries immediately while the sensor is automatically moved inside the sample.
Technical
| Specifications* | Gaseous & Dissolved O2 | Dissolved O2 |
|---|---|---|
| *data for microsensor TF = flat-broken sensor tip with 230 μm diameter **after two-point calibration as described in the manual | ||
| Measurement range | 0 – 100 % O2 0 – 1000 hPa | 0 – 45 mg/L 0 – 1400 µmol/L |
| Limit of detection | 0.03 % oxygen | 15 ppb |
| Resolution | ± 0.01 % O2 at 1 % O2 | ± 0.005 mg/L at 0.4mg/L ± 0.025 mg/L at 9.06 mg/L |
| Accuracy at + 20 °C** | ± 0.05 % O2 or ± 3 % rel. | |
| Measurement temperature range | from 0 to + 50 °C | |
| Response time (t90) | < 3 sec. (gas) | < 10 sec. (liquid) |
| Properties | ||
| Compatibility | Aqueous solutions, ethanol, methanol | |
| No cross-sensitivity | pH 1 – 14 | |
| Cross-sensitivity | Organic solvents, such as acetone, toluene, chloroform or methylene chloride | |
| Sterilization procedure | Ethylene oxide (EtO) | |
| Cleaning procedure | 3 % H2O2 | |
| Calibration | Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment | |
| Storage stability | 60 months provided the sensor material is stored in the dark at room temperature | |
Related products
Resources
Publications
High Resolution O2 Microprofiling of a Freshwater Stream Biofilm
O2, pH and CO2 Dynamics in Salt Marsh Tidal Ponds
Oxygen Gradients in Collagen-I Gels
Electrocatalytic on-site oxygenation for transplanted cell-based-therapies
Automated 3D Microphysiometry Facilitates High-Content and Highly Reproducible Oxygen Measurements within 3D Cell Culture Models
Erythropoietin Abrogates Post-Ischemic Activation of the NLRP3, NLRC4, and AIM2 Inflammasomes in Microglia/Macrophages in a TAK1-Dependent Manner
When water returns: Drying history shapes respiration and nutrients release of intermittent river sediment
Shallow Salt Marsh Tidal Ponds - An Environment with Extreme Oxygen Dynamics
The effect of lake browning and respiration mode on the burial and fate of carbon and mercury in the sediment of two boreal lakes
Oxygen-distribution within 3-D collagen I hydrogels for bone tissue engineering
FAQs
Can I change the cannula on my Profiling Microsensor?
Can I measure inside a tissue with fiber optic microsensors?
Can the Profiling Microsensor be submerged in water?
Can the Profiling Microsensor be used with the Microx TX3 (trace)?
How do I import data into excel from a text file containing measurement data?
How do I verify whether the oxygen sensor is giving correct readings (performance proof)?
How does an oxygen sensor work?
How does salinity affect the oxygen measurement?
How does temperature affect the oxygen measurement?
I can see that about 1 mm of the tapered sensor tip of an oxygen microsensor is coated with material. Is all of this coating sensitive to oxygen and where exactly do I measure?
The needle tip of the microsensor is vibrating or not moving smoothly when I try to insert it in the sample with the Manual Micromanipulator. What can I do?
The Stern-Volmer-equation
What cannula / steel needle sizes are available for Profiling Microsensors?
What is the correct way for handling an oxygen or pH microsensor?
What is the difference between tapered and flat-broken microsensor tips?
What is the smallest tip size available for Profiling Microsensors?
What is the time of delivery?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Manuals
Brochures
Software
PreSens Profiling Studio version 1.1.0 - Windows 7/8/10
PreSens Oxygen Calculator version 3.1.1 - Windows 7/8/10