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Disposable 24 Well Plate for Online pH Monitoring
HydroDish® HD24
The HydroDish® HD24 is a disposable polystyrene low-well multidish. Pre-calibrated pH sensors are integrated at the bottom of each round well and are read out contactless with the SDR SensorDish® Reader through the transparent bottom of the dish. They are delivered beta-irradiated.
- Parallel online pH monitoring
- Ready-to-use
- Pre-calibrated
- Manual calibration possible
- Ideal for cell cultivation & tissue engineering
Applications
Process Monitoring with the SDR in Suspension-Adapted CHO Cell Cultures
Oxygen and pH kinetics of suspension-adapted CHO cells at different start concentrations were monitored. The kinetics correlated well with the initial cell concentrations. pH values decreased only to a minimum of 6.3 due to daily media change. Samples with the lowest cell concentration did not show any pH change due to lack of cell growth. The oxygen uptake rate increased at each medium change. For the highest initial cell concentration it decreased at the end of the cultivation due to forming of aggregates. The results were confirmed by microscopic investigation.
Dr. Harry Abts, Celonic GmbH, Jülich, Germany, BioProcess Int., Jan 2008: 64-66
pH Monitoring in 3D Culture of Disc-Chodrocytes
Hydrogel-based 3D cell culture systems offer particular advantages for tissue-engineering in
cell-based therapy for disc-degeneration. pH monitoring at different start cell densities was conducted in HydroDishes®. pH kinetics revealed characteristic dynamics according to the length of cultivation and the initial cell density. The pH values decreased without falling below pH 7.0 (Crabtree effect). At higher initial cell densities it took only about one day in order to reach the respective pH minimum. Due to the change of culture medium, a brief pH shift beyond physiologically optimal pH values took place. Removing the culture from the controlled incubator atmosphere (indicated by *) also resulted in a short increase in pH value, which could be recorded with the SDR.
K. Benz, et al., Regenerative Medicine II, NMI Reutlingen, Germany
Technical
| Specifications | |
|---|---|
| * in physiological solutions, 37 °C | |
| Measurement range | pH 6.0 - 8.5 |
| Resolution* | at pH = 7: ± 0.05 pH |
| Precision* | ± 0.2 pH at pH = 7 (sensor batch calibration) ± 0.1 pH at pH = 7 (sensor spot calibration) |
| Drift* | < 0.1 pH within one week (sampling interval 10 min.) |
| Measurement temperature range | from + 15 °C to + 45 °C |
| Response time* (t90) | at 25 °C: < 120 sec. |
| Properties | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10 |
| Cross-sensitivity | Reduced to ionic strength (salinity); high concentration of small fluorescent molecules in the visible range can interfere |
| Calibration | Pre-calibrated Disposables are delivered beta-irradiated |
| Maximum filling volume | 3 mL |
Related products
Resources
Publications
Quality Assessment of Primary Human Keratinocyte Cultures
Process Monitoring in Suspension-Adapted CHO Cell Cultures
3D Cultures of Disc-Chondrocytes
Design of Experiment (DoE) for Fed-batch Process Development in Shaken Cultures
Characterization of Oxygen Levels in an Uninfected and Infected Human Blood-Cerebrospinal-Fluid-Barrier Model
Acidic microenvironments Found in Cutaneous Leishmania Lesions Curtail NO-Dependent Antiparasitic Macrophage Activity
Characterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter plates
Pioglitazone modulates tumor cell metabolism and proliferation in multicellular tumor spheroids
Monitoring pH and dissolved oxygen in mammalian cell culture using optical sensors
Interference of magnesium corrosion with tetrazolium-based cytotoxicity assays
FAQs
Can I re-use the SensorDishes®?
Can I use the SDR with other formats than the 24-well plates?
Do cells grow on the sensor inside the SensorDishes®?
Do SensorDishes® come sterile?
How do I import data into excel from a text file containing measurement data?
How long can I use a SensorDish®?
How long do I need to equilibrate before starting a SDR measurement?
The SDR software shows "no sensor" instead of a value. What is the reason?
What is the difference between User-Defined Calibration and One-Point Adjustment for the SDR? When do I apply which one?
What is the time of delivery?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Which toxicity tests where done with the SensorDish®?
Why are there two different calibration data sets for SensorDishes®?
Why do I need to equilibrate my samples for such long time periods before measurement with the SDR?
Why do I see peaks in the graphs of the SDR software (especially in the oxygen signal) when I open the incubator door / take the plates out of the incubator?
Why does the SDR software show pH<5, although I know that my sample is basic?