Disposable 24-Well Plate for Online Oxygen Monitoring

OxoDish® OD24

The OxoDish® OD24 is a disposable polystyrene low-well multidish. Pre-calibrated oxygen sensors are integrated at the bottom of each well and are read out contactless through the transparent well bottom with the SDR SensorDish® Reader. The OxoDishes® are delivered beta-irradiated.

  • Parallel online oxygen measurements
  • Ready-to-use
  • Pre-calibrated
  • Manual calibration possible
  • For microbial, tissue & cell cultures
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More Security at Hypoxic Stem Cell Cultivation

The influence of medium change on dissolved oxygen (DO) at cultivation of human embryonic stem cells (hESC) was investigated at different oxygen tensions in the incubator atmosphere. Samples with full medium change using non-precalibrated medium showed a DO increase of 20 - 60 % air saturation. Other than expected, even half medium change with pre-incubated medium resulted in a notable DO increase of 10 - 30 % air saturation. The SensorDish® Reader can be used in hypoxia incubators, but also in small hypoxia chambers (see picture).

Barbara Ley, Prof. Oliver Brüstle, Life & Brain GmbH, Bonn, Germany

Oxygen and pH Monitoring in Tissue Engineering

Human chondrocytes with different start cell concentrations were cultivated in OxoDishes® and HydroDishes®. pH deviations from the control wells (medium only) could be detected even for the lowest start concentration. The acidification rates were in accordance with the different start concentrations. Medium change after 5 days led to a temporal pH increase until the samples were in equilibrium with the incubator atmosphere (5% CO2) again. Oxygen kinetics show the respective oxygen decrease.

Dr. Andreas Thomsen, CellGenix GmbH, Freiburg, Germany

High Throughput Screening of Water Oxidation Reactions with OxoDishes®

Splitting of water in a light-driven reaction is one of the most promising processes for producing clean fuel. In order to create an efficient process suitable reaction catalysts must be found. A high throughput screening of more than 200 reactions with different catalysts was performed using OxoDishes® and oxygen production was recorded. The example shows oxygen monitoring of complex 1 at different concentrations under different reaction conditions. The OxoDishes® and the SDR proved very useful for high throughput analyses and several catalysts that could form oxygen at the used reaction conditions could be identified.

R. J. Detz1, Z. Abiri1,2, A. M. Kluwer2, J. N. H. Reek1, 1Van't Hoff Institute for Molecular Sciences, University of Amsterdam, NL, 2InCatT B. V., Amsterdam, NL


* in H2O dest. or oxygen-free water
Measurement range 0 - 50 % O2
Resolution* ± 0.4 % O2
Precision* ± 1 % O2
Drift* < 0.2 % O2 within one week (sampling interval 10 min.)
Measurement temperature range from + 15 to + 45 °C
Response time* (t90) at + 25 °C: < 30 sec.
Compatibility Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10
Maximum filling volume 3 mL
Calibration Pre-calibrated
  SensorDishes® are delivered beta-irradiated

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Quality Assessment of Primary Human Keratinocyte Cultures Energy Catabolism Measurements in Metastatic Macrophage M3 Cells Identifying Water Oxidation Catalysts for Clean Fuel Production Localized Oxygen Depletion by Transmigrating Neutrophils Influences Inflammatory Resolution Correcting for Oxygen Diffusion in Respiration Measurements Non-Invasive Online Oxygen Measurement Process Monitoring in Suspension-Adapted CHO Cell Cultures Online Oxygen Monitoring System A Novel Online Monitoring System 3D Cultures of Disc-Chondrocytes Online Oxygen Monitoring in Cell Culture Design of Experiment (DoE) for Fed-batch Process Development in Shaken Cultures High Throughput Respiration Assay for Cytotoxicity Determination Non-invasive Method for Monitoring Real-Time Oxygen during Hematopoietic Stem/Progenitor Cell (HSPC) Culture Real Time Respiration Measurements Toxicological effects of 6PPD and 6PPD quinone in zebrafish larvae Phytoplankton diversity affects biomass and energy production differently during community development Energetic mismatch induced by warming decreases leaf litter decomposition by aquatic detritivores Teleocidin-producing genotype of Streptomyces clavuligerus ATCC 27064 Characterization of Oxygen Levels in an Uninfected and Infected Human Blood-Cerebrospinal-Fluid-Barrier Model An Inexpensive Incubator for Mammalian Cell Culture Capable of Regulating O2, CO2, and Temperature Characterization of the biological sulfide oxidation process: in situ pulse respirometry and ex situ pulse microrespirometry approach Small-scale hypoxic cultures for monitoring the spatial reorganization of glycolytic enzymes in Saccharomyces cerevisiae Restricting Glycolysis Preserves T Cell Effector Functions and Augments Checkpoint Therapy Characterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter plates High troughput, non-invasive and dynamic toxicity screening on adherent cells using respiratory measurements Pioglitazone modulates tumor cell metabolism and proliferation in multicellular tumor spheroids Lactic Acid and Acidification Inhibit TNF Secretion and Glycolysis of Human Monocytes Monitoring pH and dissolved oxygen in mammalian cell culture using optical sensors Analysis of OPLA scaffolds for bone engineering constructs using human jaw periosteal cells Chemoradiation interactions under reduced oxygen conditions: Cellular characteristics of an in vitro model High glucose concentrations attenuate hypoxia-inducible factor-1α expression and signaling in non-tumor cells Reduced oxygen stress promotes propagation of murine postnatal enteric neural progenitors in vitro Adaptation to oxygen deprivation in cultures of human pluripotent stem cells, endothelial progenitor cells, and umbilical vein endothelial cells Different stages of pluripotency determine distinct patterns of proliferation, metabolism, and lineage commitment of embryonic stem cells under hypoxia Oxygen requirements of zebrafish (Danio rerio) embryos in embryo toxicity tests with environmental samples Unforeseen decrease in dissolved oxygen levels affect tube formation kinetics in collagen gels Non-invasive determination of cellular oxygen consumption as novel cytotoxicity assay for nanomaterials Respirometry: Correcting for Diffusion and Validating the Use of Plastic Multiwell Plates with Integrated Optodes Measuring dissolved oxygen to track erythroid differentiation of hematopoietic progenitor cells in culture A Fluorescence-Based Screening Protocol for the Identification of Water Oxidation Catlysts

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