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Disposable 24-Well Plate for Online Oxygen Monitoring
OxoDish® OD24
The OxoDish® OD24 is a disposable polystyrene low-well multidish. Pre-calibrated oxygen sensors are integrated at the bottom of each well and are read out contactless through the transparent well bottom with the SDR SensorDish® Reader. The OxoDishes® are delivered beta-irradiated.
- Parallel online oxygen measurements
- Ready-to-use
- Pre-calibrated
- Manual calibration possible
- For microbial, tissue & cell cultures
Applications
More Security at Hypoxic Stem Cell Cultivation
The influence of medium change on dissolved oxygen (DO) at cultivation of human embryonic stem cells (hESC) was investigated at different oxygen tensions in the incubator atmosphere. Samples with full medium change using non-precalibrated medium showed a DO increase of 20 - 60 % air saturation. Other than expected, even half medium change with pre-incubated medium resulted in a notable DO increase of 10 - 30 % air saturation. The SensorDish® Reader can be used in hypoxia incubators, but also in small hypoxia chambers (see picture).
Barbara Ley, Prof. Oliver Brüstle, Life & Brain GmbH, Bonn, Germany
Oxygen and pH Monitoring in Tissue Engineering
Human chondrocytes with different start cell concentrations were cultivated in OxoDishes® and HydroDishes®. pH deviations from the control wells (medium only) could be detected even for the lowest start concentration. The acidification rates were in accordance with the different start concentrations. Medium change after 5 days led to a temporal pH increase until the samples were in equilibrium with the incubator atmosphere (5% CO2) again. Oxygen kinetics show the respective oxygen decrease.
Dr. Andreas Thomsen, CellGenix GmbH, Freiburg, Germany
High Throughput Screening of Water Oxidation Reactions with OxoDishes®
Splitting of water in a light-driven reaction is one of the most promising processes for producing clean fuel. In order to create an efficient process suitable reaction catalysts must be found. A high throughput screening of more than 200 reactions with different catalysts was performed using OxoDishes® and oxygen production was recorded. The example shows oxygen monitoring of complex 1 at different concentrations under different reaction conditions. The OxoDishes® and the SDR proved very useful for high throughput analyses and several catalysts that could form oxygen at the used reaction conditions could be identified.
R. J. Detz1, Z. Abiri1,2, A. M. Kluwer2, J. N. H. Reek1, 1Van't Hoff Institute for Molecular Sciences, University of Amsterdam, NL, 2InCatT B. V., Amsterdam, NL
Technical
| Specifications | |
|---|---|
| * in H2O dest. or oxygen-free water | |
| Measurement range | 0 - 50 % O2 |
| Resolution* | ± 0.4 % O2 |
| Precision* | ± 1 % O2 |
| Drift* | < 0.2 % O2 within one week (sampling interval 10 min.) |
| Measurement temperature range | from + 15 to + 45 °C |
| Response time* (t90) | at + 25 °C: < 30 sec. |
| Properties | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10 |
| Maximum filling volume | 3 mL |
| Calibration | Pre-calibrated |
| SensorDishes® are delivered beta-irradiated | |
Related products
Resources
Publications
Quality Assessment of Primary Human Keratinocyte Cultures
Energy Catabolism Measurements in Metastatic Macrophage M3 Cells
Identifying Water Oxidation Catalysts for Clean Fuel Production
Localized Oxygen Depletion by Transmigrating Neutrophils Influences Inflammatory Resolution
Correcting for Oxygen Diffusion in Respiration Measurements
Non-Invasive Online Oxygen Measurement
Process Monitoring in Suspension-Adapted CHO Cell Cultures
Online Oxygen Monitoring System
A Novel Online Monitoring System
3D Cultures of Disc-Chondrocytes
Online Oxygen Monitoring in Cell Culture
Design of Experiment (DoE) for Fed-batch Process Development in Shaken Cultures
High Throughput Respiration Assay for Cytotoxicity Determination
Non-invasive Method for Monitoring Real-Time Oxygen during Hematopoietic Stem/Progenitor Cell (HSPC) Culture
Real Time Respiration Measurements
Toxicological effects of 6PPD and 6PPD quinone in zebrafish larvae
Phytoplankton diversity affects biomass and energy production differently during community development
Energetic mismatch induced by warming decreases leaf litter decomposition by aquatic detritivores
Teleocidin-producing genotype of Streptomyces clavuligerus ATCC 27064
Characterization of Oxygen Levels in an Uninfected and Infected Human Blood-Cerebrospinal-Fluid-Barrier Model
An Inexpensive Incubator for Mammalian Cell Culture Capable of Regulating O2, CO2, and Temperature
Characterization of the biological sulfide oxidation process: in situ pulse respirometry and ex situ pulse microrespirometry approach
Small-scale hypoxic cultures for monitoring the spatial reorganization of glycolytic enzymes in Saccharomyces cerevisiae
Restricting Glycolysis Preserves T Cell Effector Functions and Augments Checkpoint Therapy
Characterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter plates
High troughput, non-invasive and dynamic toxicity screening on adherent cells using respiratory measurements
Pioglitazone modulates tumor cell metabolism and proliferation in multicellular tumor spheroids
Lactic Acid and Acidification Inhibit TNF Secretion and Glycolysis of Human Monocytes
Monitoring pH and dissolved oxygen in mammalian cell culture using optical sensors
Analysis of OPLA scaffolds for bone engineering constructs using human jaw periosteal cells
Chemoradiation interactions under reduced oxygen conditions: Cellular characteristics of an in vitro model
High glucose concentrations attenuate hypoxia-inducible factor-1α expression and signaling in non-tumor cells
Reduced oxygen stress promotes propagation of murine postnatal enteric neural progenitors in vitro
Adaptation to oxygen deprivation in cultures of human pluripotent stem cells, endothelial progenitor cells, and umbilical vein endothelial cells
Different stages of pluripotency determine distinct patterns of proliferation, metabolism, and lineage commitment of embryonic stem cells under hypoxia
Oxygen requirements of zebrafish (Danio rerio) embryos in embryo toxicity tests with environmental samples
Unforeseen decrease in dissolved oxygen levels affect tube formation kinetics in collagen gels
Non-invasive determination of cellular oxygen consumption as novel cytotoxicity assay for nanomaterials
Respirometry: Correcting for Diffusion and Validating the Use of Plastic Multiwell Plates with Integrated Optodes
Measuring dissolved oxygen to track erythroid differentiation of hematopoietic progenitor cells in culture
A Fluorescence-Based Screening Protocol for the Identification of Water Oxidation Catlysts
FAQs
Can I re-use the SensorDishes®?
Can I use the SDR with other formats than the 24-well plates?
Do cells grow on the sensor inside the SensorDishes®?
Do SensorDishes® come sterile?
How can I convert an oxygen value into a different oxygen unit?
How do I import data into excel from a text file containing measurement data?
How does temperature affect the oxygen measurement?
How long can I use a SensorDish®?
How long do I need to equilibrate before starting a SDR measurement?
The SDR software shows "no sensor" instead of a value. What is the reason?
What is the difference between User-Defined Calibration and One-Point Adjustment for the SDR? When do I apply which one?
What is the time of delivery?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Which toxicity tests where done with the SensorDish®?
Why are there two different calibration data sets for SensorDishes®?
Why do I need to equilibrate my samples for such long time periods before measurement with the SDR?
Why do I see peaks in the graphs of the SDR software (especially in the oxygen signal) when I open the incubator door / take the plates out of the incubator?