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Robust Microsensor for Oxygen Profiling Applications in Semi-Solid Samples

Profiling Oxygen Microsensor PM-PSt1

The profiling oxygen microsensor PM-PSt1 is compatible with the discontinued Microx TX3 / Microx TX3 trace oxygen meters. It has a robust splash-proof metal housing. The optical fiber and the tiny sensor tip are protected inside a steel cannula and can be extended with a turning mechanism. This microsensor is the sturdiest version of PreSens microsensors, and can be applied for profiling inside semi-solid samples like sediments, biofilm or microbial mats. The sensor has a mechanical interlock which enables exact localization of the sensor tip inside the sample. Together with the Automated Micromanipulator it is a perfect oxygen profiling tool.

  • High spatial resolution (< 50 μm)
  • High temporal resolution (t90 < 3 sec.)
  • Penetration probe for insertion into semi-solids
  • Mechanical interlock for precise vertical localization inside the sample
  • Splash-proof metal housing
  • No electrical interference due to optical measurements

Applications

Microprofiling in Biological & Environmental Research

The different types of oxygen and pH microsensors allow e. g. measurements in smallest sample volumes or inside tissue. The micromanipulators should be applied whenever it is necessary to insert the microsensor safely into semi-solid samples and when exact localization and stabilization of the microsensor tip within the sample is required. Using the safe-insert function the microsensor tip can be securely inserted and localized at the exact position where you want to conduct your measurements.


Microsensor Measurements in Medical & Life Science Research

PreSens microsensors are ideal tools for medical and life science research, as they allow for precise on the spot measurement and microprofiling inside tissue constructs. The Manual Micromanipulator is the indispensable equipment in these applications for exact localization of the microsensor inside the sample and microprofiling in step sizes down to 10 μm. PreSens needle-type microsensors are used in many tissue engineering applications.


Microprofiling of Sediments & Biofilms

Together with the specially designed PreSens Profiling Microsensors (PM) the Automated Micromanipulator is the ideal tool for oxygen measurements in sediment and biofilm applications. With a free choice of step zones, travel velocities and wait times different layers inside the sample can be monitored and assessed in step sizes down to 10 μm. The software visualizes the online measurements, so you can follow gradients and identify boundaries immediately while the sensor is automatically moved inside the sample.


Technical

SpecificationsGaseous & Dissolved O2Dissolved O2

*after two-point calibration as described in the manual
**TS: tapered sensor tip with a diameter < 50 μm and no optical isolation
***TF: flat-broken sensor tip with a diameter of 140 μm

Measurement range

0 – 50 % O2

0 – 500 hPa

0 – 22.5 mg/L

0 – 700 µmol/L
Limit of detection0.05 % oxygen20 ppb
Resolution

± 0.01 % O2 at 0.21 % O2
± 0.09 % O2 at 20.9 % O2
± 0.1 hPa at 2 hPa

± 1 hPa at 207 hPa

± 0.005 mg/L at 0.09 mg/L
± 0.04 mg/L at 9.06 mg/L
± 0.14 µmol/L at 2.83 µmol/L

± 1.3 µmol/L at 283 µmol/L
Accuracy*± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2
Drift< 0.1 % O2 within 30 days (sampling interval of 1 min.)
Measurement temperature rangefrom 0 to + 50 °C
Response time TS** (t90)< 1 sec. (gas)< 2 sec. (liquid)
Response time TF*** (t90)< 15 sec. (gas)< 30 sec. (liquid)
Properties
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivityOrganic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure

Ethylene oxide (EtO)

Cleaning procedure3 % H2O2
Ethanol
Soap solution
CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability60 months provided the sensor material is stored in the dark at room temperature

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