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Precise Oxygen Measurements under Real Conditions

Oxygen Sensor Spot SP-PSt3-NAU

Sensor spots are the most versatile version of non-invasive optical oxygen sensors. The red side of the spot can be attached to the inner surface of any transparent glass or plastic vessel like e. g. shake and spinner flasks, tubes, Petri dishes or cultivation bags. Oxygen is measured contactless and non-destructively through the transparent vessel wall. The SP-PSt3-NAU has a measurement range of 0 – 100 % oxygen in dissolved or gaseous phase. The oxygen sensitive coating is immobilized on 125 µm flexible transparent polyester foil, which does not stand autoclaving.

  • Non-invasive measurements through the vessel wall
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measure oxygen in liquids as well as in gas phase

Applications

Bioprocess Development: Oxygen Monitoring in Shake Flasks

O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.


Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Technical

SpecificationsGaseous & Dissolved O2Dissolved O2
*after two-point calibration as described in the manual
Measurement range

0 – 100 % O2

0 – 1000 hPa

0 – 45 mg/L

0 – 1400 µmol/L
Limit of detection0.03 % oxygen15 ppb
Resolution

± 0.01 % O2 at 0.21 % O2
± 0.1 % O2 at 20.9 % O2
±0.1 hPa at 2 hPa

± 1 hPa at 207 hPa

± 0.004 mg/L at 0.091 mg/L
± 0.04 mg/L at 9.1 mg/L
± 0.14 µmol/L at 2.83 µmol/L

± 1.4 µmol/L at 283.1 µmol/L
Accuracy*± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2
Drift< 0.03 % O2 within 30 days (sampling interval of 1 min. / at 0% oxygen)
Measurement temperature rangefrom 0 to + 50 °C
Response time (t90)< 6 sec.< 40 sec.
Properties
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivityOrganic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedureEthylene oxide (EtO)
Gamma irradiation
Cleaning procedure Cleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 – 5 %
CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability24 months provided the sensor material is stored in the dark

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Resources

Publications

Quantification of Oxygen Consumption of Meat-Spoiling Microorganisms below 30 % CO2 / 70 % O2 Atmosphere Measuring Dissolved O2 in Dimethyl Sulfoxide Solution in the Presence of Different Lithium Salts Simultaneous pH, CO2 and O2 Measurements in Algae Photobioreactor Advanced Respirometry with Chemical Optical Sensor Spots Constant Oxygen Monitoring during 13 Days on Satellite Mission in Outer Space Online pH and DO Measurements in Microcarrier-Based hMSC Cultivations Studying Microbial Iron and Manganese Oxidation in Different Experimental Set-Ups Online in Situ Measurements of Soil CO2 Concentrations in Dependence of Ash Roots (Fraxinus excelsior L.) Biogeochemical gradients and microbial communities in Winogradsky columns established with polluted wetland sediments Contribution of Microaerophilic Iron(II)-Oxidizers to Iron(III) Mineral Formation Aerobic and oxygen-limited enrichment of BTEX-degrading biofilm bacteria: dominance of Malikia versus Acidovorax species Transcriptome-Stable Isotope Probing Provides Targeted Functional and Taxonomic Insights Into Microaerobic Pollutant-Degrading Aquifer Microbiota Stable isotope probing for hypoxic toluene degradation at the Siklós aquifer reveals prominent role of Rhodocyclaceae In situ measurement of oxygen consumption to estimate corrosion rates Oxic Fe(III) reduction could have generated Fe(II) in the photic zone of Precambrian seawater Turbulence induces metabolically costly behaviors and inhibits food capture in oyster larvae, causing net energy loss Comparison of test methods for oxygen permeability: Optical method versus carrier gas method Production of functionalized oligo-isoprenoids by enzymatic cleavage of rubber Structural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of Rubber RoxB Is a Novel Type of Rubber Oxygenase That Combines Properties of Rubber Oxygenase RoxA and Latex Clearing Protein (Lcp) Effects of ascorbic acid and light on reactions in fresh-cut apples by microcalorimetry Assays for the Detection of Rubber Oxygenase Activities Dissolved Organic Nitrogen Inputs from Wastewater Treatment Plant Effluents Increase Responses of Planktonic Metabolic Rates to Warming Effects of wastewater treatment plant effluent inputs on planktonic metabolic rates and microbial community composition in the Baltic Sea Effect of exposure to sunlight and phosphorus-limitation on bacterial degradation of coloured dissolved organic matter (CDOM) in freshwater Competition between Oxygen and Nitrate Respirations in Continous Culture of Pseudomonas aeruginosa Performing Aerobic Denitrification Effects of Iron on Chemical Sulfide Oxidation in Wastewater from Sewer Networks Kinetics of chlorobenzene biodegradation under reduced oxygen levels Adult neural stem cells express glucose transporters GLUT1 and GLUT3 and regulate GLUT3 expression Aerated subsurface irrigation water gives growth and yield benefits to zucchini, vegetable soybean and cotton in heavy clay soils New and Fast Method To Quantify Respiration Rates of Bacterial and Plankton Communities in Freshwater Ecosystems by Using Optical Oxygen Sensor Spots Oxygen consumption of a single embryo/planula in the reef-building coral Acropora intermedia Biomass-specific respiration rates of benthic meiofauna: Demonstrating a novel oxygen micro-respiration system Chlorobenzene biodegradation under consecutive aerobic-anaerobic conditions Essential fatty acids influence metabolic rate and tolerance of hypoxia in Dover sole (Solea solea) larvae and juveniles Evaluation of a lifetime-based optode to measure oxygen in aquatic systems A promising environmentally-friendly manganese-based catalyst for alkyd emulsion coatings A Novel Method to Determine Oxidation Rates of Heritage Materials in Vitro and in Situ Niche specialization of reef-building corals in the mesophotic zone: metabolic trade-offs between divergent Symbiodinium types Magnitude and regulation of bacterioplankton respiratory quotient across feshwater environmental gradients A high-resolution non-invasive approach to quantify oxygen transport across the capillary fringe and within the underlying groundwater Aquatic Respiration Rate Measurements at Low Oxygen Concentrations Zoogloea oleivorans sp. nov., a floc-forming, petroleum hydrocarbon-degrading bacterium isolated from biofilm Vadose zone oxygen (O2) dynamics during drying and wetting cycles: An artificial recharge laboratory experiment Influence of plasticizers on the mechanical and barrier properties of cast biopolymer films Experimental Sensitivity Analysis of Oxygen Transfer in the Capillary Fringe Connecting bacterial colonization to physical and biochemical changes in a sand box infiltration experiment Oxygen Transfer in a Fluctuating Capillary Fringe: Impact of Microbial Respiratory Activity Metabolic rates of a hypogean and an epigean species of copepod in an alluvial aquifer Comparison of an Electrochemical and Luminescence-Based Oxygen Measuring System for Use in the Biodegradability Testing According to Closed Bottle Test (OECD 301D)

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