Precise Oxygen Measurements under Real Conditions

Oxygen Sensor Spot SP-PSt3-NAU

Sensor spots are the most versatile version of non-invasive optical oxygen sensors. The red side of the spot can be attached to the inner surface of any transparent glass or plastic vessel like e. g. shake and spinner flasks, tubes, Petri dishes or cultivation bags. Oxygen is measured contactless and non-destructively through the transparent vessel wall. The SP-PSt3-NAU has a measurement range of 0 – 100 % oxygen in dissolved or gaseous phase. The oxygen sensitive coating is immobilized on 125 µm flexible transparent polyester foil, which does not stand autoclaving.

  • Non-invasive measurements through the vessel wall
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measure oxygen in liquids as well as in gas phase
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Bioprocess Development: Oxygen Monitoring in Shake Flasks

O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.

Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Specifications Gaseous & Dissolved O2 Dissolved O2
*after two-point calibration as described in the manual
Measurement range

0 – 100 % O2

0 – 1000 hPa

0 – 45 mg/L

0 – 1400 µmol/L
Limit of detection 0.03 % oxygen 15 ppb

± 0.01 % O2 at 0.21 % O2
± 0.1 % O2 at 20.9 % O2
±0.1 hPa at 2 hPa

± 1 hPa at 207 hPa

± 0.004 mg/L at 0.091 mg/L
± 0.04 mg/L at 9.1 mg/L
± 0.14 µmol/L at 2.83 µmol/L

± 1.4 µmol/L at 283.1 µmol/L
Accuracy* ± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2
Drift < 0.03 % O2 within 30 days (sampling interval of 1 min. / at 0% oxygen)
Measurement temperature range from 0 to + 50 °C
Response time (t90) < 6 sec. < 40 sec.
Compatibility Aqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivity Organic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure Ethylene oxide (EtO)
Gamma irradiation
Cleaning procedure Cleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 – 5 %
Aqueous solutions
Calibration Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability 60 months provided the sensor material is stored in the dark

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Disentangling the Drivers of Benthic Oxygen and Dissolved Carbon Fluxes in the Coastal Zone of the Southern Baltic Sea Fluctuation at High Temperature Combined with Nutrients Alters the Thermal Dependence of Phytoplankton Large differences in bacterial community composition of nearby shallow lakes surrounded by Nothofagus pumilio forest in Patagonia (Argentina) Pinisolibacter aquiterrae sp. nov., a novel aromatic hydrocarbon-degrading bacterium isolated from benzene-, and xylene-degrading enrichment cultures, and emended description of the genus Pinisolibacter Desert dust deposition supplies essential bioelements to Red Sea corals Responsiveness to contest experiences is associated with competitive ability but not aggressiveness or boldness Non-Destructive Measuring Systems for the Evaluation of High Oxygen Stored Poultry: Development of Headspace Gas Composition, Sensory and Microbiological Spoilage The short and long‑term implications of warming and increased sea water pCO2 on the physiological response of a temperate neogastropod species Response of Cymodocea nodosa to ocean acidification and warming in the Canary Islands: Direct and indirect effects Plasticity to ocean warming is influenced by transgenerational, reproductive, and developmental exposure in a coral reef fish Analyses of metabolic activity in peanuts under hermetic storage at different relative humidity levels Protist Predation Influences the Temperature Response of Bacterial Communities Effects of temperature and oxygen on 137Cs desorption from bottom sediment of a dam lake Metabolite Profiling in Green Microalgae with Varying Degrees of Desiccation Tolerance Effect of ozonation on the biodegradability of urban wastewater treatment plant effluent High-latitude calcified coralline algae exhibit seasonal vulnerability to acidification despite physical proximity to a non-calcified alga Transient behavior of arsenic in vadose zone under alternating wet and dry conditions: A comparative soil column study Transcriptomic analysis of chloride tolerance in Leptospirillum ferriphilum DSM 14647 adapted to NaCl The role of methanotrophy in the microbial carbon metabolism of temperate lakes Evolutionary constraints on physiology confound range shift predictions of two nacellid limpets Microbial plankton responses to multiple environmental drivers in marine ecosystems with different phosphorus limitation degrees Toward Improved Bioremediation Strategies: Response of BAM-Degradation Activity to Concentration and Flow Changes in an Inoculated Bench-Scale Sediment Tank Effect of water salinity on the oxidative system of juveniles of the North Atlantic white shrimp Litopenaeus setiferus reared in biofloc technology Growth of microaerophilic Fe(II)-oxidizing bacteria using Fe(II) produced by Fe(III) photoreduction Xenobiotic metabolism and its physiological consequences in high-Antarctic Notothenioid fishes High pCO2 does not alter the thermal plasticity of developing Pacific herring embryos during a marine heatwave Hydrocarbon biodegradation potential of microbial communities from high Arctic beaches in Canada's Northwest Passage Determination of oxygen relaxivity in oxygen nanobubbles at 3 and 7 Tesla In Vivo Functional Assay in Fish Gills: Exploring Branchial Acid-Excreting Mechanisms in Zebrafish Photophysiological investigations of the temperature stress responses of Zygnema spp (Zygnematophyceae) from subpolar and polar habitats (Iceland, Svalbard) Future Climate Change Conditions May Compromise Metabolic Performance in Juveniles of the Mud Crab Scylla serrata Portable Measurement System for in situ Estimation of Oxygen and Carbon Fluxes of Submerged Plants Seasonal time constraints shape life history, physiology and behaviour independently, and decouple a behavioural syndrome in a damselfly Development of a new high-throughput and small-size method for measuring sediment oxygen demand in lakes Anaerobic sulfur oxidation underlies adaptation of a chemosynthetic 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Do I have to mount the sensors in the dark because it says "Protect from light" on the packing? Do the sensors work in turbid solutions? Does each oxygen sensor spot need a separate calibration, or are the calibration values valid for the complete sensor spot batch? How can I convert an oxygen value into a different oxygen unit? How can I prepare the calibration solutions cal0 and cal100 for oxygen sensors? How do I import data into excel from a text file containing measurement data? How do I verify whether the oxygen sensor is giving correct readings (performance proof)? How does an oxygen sensor work? How does salinity affect the oxygen measurement? How does temperature affect the oxygen measurement? How many samples should I use to perform a batch calibration? In which vessel can I integrate a sensor spot? Is the glue or the sensor spot biocompatible - which tests were done? Are there any studies on leachables and extractables? Is there a standard recommended distance to place the polymer optical fiber (POF) from the sensor spot? The Stern-Volmer-equation What are the response times for the oxygen sensors based on the 2 mm fiber like non-invasive oxygen sensors and oxygen probes? What does -YAU, -NAU or -SA stand for in the oxygen sensor spot names and what is the difference between those sensor types? What factors will affect the oxygen reading? What is the time of delivery? Where do I need sensor spot sizes different from the common diameter of 5 mm (approx. 0.2 inch)? Which glue can I use to integrate optical sensor spots? Which side of the oxygen sensor spot should face the medium? Which substances can interfere with the optical O2, pH and CO2 measurements?



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