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Modular O2, pH and CO2 Mapping System

VisiSens TD

VisiSens TD enables simultaneous 2D read-out of optical O2, pH and CO2 sensor foils within one set-up. Planar sensors for one or more analytes are placed on the sample area or in different cavities and the fluorescent sensor signals are read out pixel by pixel with a camera. Fields of view from mm2 up to 30 cm x 25 cm are possible. VisiSens TD gives an overview over your sample area and allows to freely choose the region of interest for investigation of spatial and temporal gradients or simultaneous read-out of multiple sensor signals. The modular system can be customized according to the users’ requirements.

  • 3 analytes – 1 system
  • Multiple sensor types combinable in one field of view
  • Variable sensor and measurement geometry
  • 12-bit detector
  • Adaptable field of view, microscopic, 4 x 4 cm2 or up to 30 x 25 cm2
  • Time-lapse slide shows of recordings

Applications

Visualizing Analytes over Large Sample Surfaces

The VisiSens TD images large areas and gathers information on spatial and temporal analyte changes, e. g. in large scale pH and CO2 imaging of root-soil interactions within rhizotrons or when analyzing biogeochemical processes in sediments. It is also well suited for application in sensitive processes, e. g. metabolic activity of living samples or tissue engineering, where O2 gradients over a cross section of a 3D graft can be monitored.


Multi-Sample and Multi-Sensor Read-Out

Large field of views enable analyte value recording in multiple samples simultaneously with free choice of measurement geometry. Even different sensor ranges can be combined. The fluorescent sensor foils can be mounted at the bottom of transparent vessels, plates or vials containing different samples and placed next to each other, e. g. in a small rack. One image taken from the bottom contains the information on analyte concentrations in a group of samples.


Three Different Analytes with one Device

The modular set-up is applicable for imaging oxygen, pH, and CO2 sensor foils with one system and in one field of view. It visualizes spatial and temporal changes of analyte concentrations. The combined information on distributions of several analytes can give valuable insight in metabolic activity or chemical processes inside samples, e. g. when investigating complex processes in cell culture, or tissues, in root systems, or when phenotyping crop plants.


Microscopic Analyte Imaging - TD MIC Configuration

VisiSens TD can be equipped with microscope optics for measuring analyte gradients with very high spatial resolution. The VisiSens TD MIC can be used e.g. to monitor spatial and temporal changes of O2 of cross-sections of multicellular tumor spheroids. The system is compact and fits into an incubator to monitor analyte changes under cell culture conditions over days.


Technical


Oxygen (blue)pH (red)CO2 (green)
SpecificationsSF-RPSu4SF-LV1RSF-HP5RSF-CD2R*SF-CD1R
Measurement range0 - 100 % air saturation (0 - 20.9 % O2)pH 2.5 - 4.5pH 5.5 - 7.50 - 1 % pCO2 at atmospheric pressure
(1013.15 hPa)
1 - 25 % pCO2 at atmospheric pressure (1013.15 hPa)
Response time (t90)**Gas phase: < 8 sec.
Dissolved: < 30 sec.
< 30 sec.< 30 sec.< 3 min.< 3 min.
Limit of detection***0.03 % air saturation
Precision (temporal)****± 0.02 % air saturation
at 0 % air saturation
± 0.1 % air saturation
at 100 % air saturation
± 0.01 pH at pH = 4± 0.01 pH at pH = 7± 0.02 % CO2
at 0.15 % CO2
± 0.02 % CO2
at 0.8 % CO2
± 0.02 % CO2
at 2% CO2
± 0.1 % CO2
at 25% CO2
Precision (spatial)*****± 1.5 % air saturation
at 0% air saturation
± 3 % air saturation
at 100 % air saturation
± 0.1 pH at pH = 4± 0.1 pH at pH = 7± 0.08 % CO2
at 0.15 % CO2
± 0.08 % CO2
at 0.8 % CO2
± 0.2 % CO2
at 2 % CO2
± 1.2 % CO2
at 25 % CO2
Properties
CompatibilityAqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10Aqueous solutions, pH 2 - 9Aqueous solutions, pH 4 - 9
General sensor temperature working rangefrom + 5 °C to + 45 °C
System Components
Ratiometric Imaging

* Prototype component; please contact our service team!
** Typical data which may strongly differ with adapting the imaging set-up to specific needs
*** Typical data of LOD of a defined ROI (> 6,000 pixels= over time in dark lab conditions at 20 °C, FoV 8 cm x 6 cm
**** Typical data of temporal variation in a defined ROI (> 6,000 pixels) over time in dark lab conditions at 20 d°C, FoV 8 cm x 6 cm
***** Typical data of spatial standard deviation in defined ROI (> 6,000 pixels) in dark lab conditions at 20 °C, FoV 8 cm x 6 cm

HardwareO2pHCO2
VisiSens TD Basic System*xxx
Big Area Imaging kit*xxx
TD MIC Configuration*x
Software
VisiSens ScientifiCal*
Imaging Modality O2*x
Imaging Modality pH*x
Imaging Modality CO2*x
Mixed dual or triple modes*xxx
Sensor
SF-RPSu4x
SF-HP5Rx
SF-LV1Rx
SF-CD1Rx
SF-CD2R*x
Accessories (optional)
CaliPlate - pH Calibration Helper Plate
Mounting Rack*
Sensor Plate Tubus Adapter*
Resolution Test Chart*
Plates with Integrated Sensor Foils*

 

 

 

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