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Precise Oxygen Measurements under Real Conditions

Oxygen Sensor Spot SP-PSt3-NAU

Sensor spots are the most versatile version of non-invasive optical oxygen sensors. The red side of the spot can be attached to the inner surface of any transparent glass or plastic vessel like e. g. shake and spinner flasks, tubes, Petri dishes or cultivation bags. Oxygen is measured contactless and non-destructively through the transparent vessel wall. The SP-PSt3-NAU has a measurement range of 0 – 100 % oxygen in dissolved or gaseous phase. The oxygen sensitive coating is immobilized on 125 µm flexible transparent polyester foil, which does not stand autoclaving.

  • Non-invasive measurements through the vessel wall
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measure oxygen in liquids as well as in gas phase

Applications

Bioprocess Development: Oxygen Monitoring in Shake Flasks

O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.


Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Technical

SpecificationsGaseous & Dissolved O2Dissolved O2
*after two-point calibration as described in the manual
Measurement range

0 – 100 % O2

0 – 1000 hPa

0 – 45 mg/L

0 – 1400 µmol/L
Limit of detection0.03 % oxygen15 ppb
Resolution

± 0.01 % O2 at 0.21 % O2
± 0.1 % O2 at 20.9 % O2
±0.1 hPa at 2 hPa

± 1 hPa at 207 hPa

± 0.004 mg/L at 0.091 mg/L
± 0.04 mg/L at 9.1 mg/L
± 0.14 µmol/L at 2.83 µmol/L

± 1.4 µmol/L at 283.1 µmol/L
Accuracy*± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2
Drift< 0.03 % O2 within 30 days (sampling interval of 1 min. / at 0% oxygen)
Measurement temperature rangefrom 0 to + 50 °C
Response time (t90)< 6 sec.< 40 sec.
Properties
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivityOrganic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedureEthylene oxide (EtO)
Gamma irradiation
Cleaning procedure Cleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 – 5 %
CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability24 months provided the sensor material is stored in the dark

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Resources

Publications

Measuring Dissolved O2 in Dimethyl Sulfoxide Solution in the Presence of Different Lithium SaltsSimultaneous pH, CO2 and O2 Measurements in Algae PhotobioreactorAdvanced Respirometry with Chemical Optical Sensor SpotsConstant Oxygen Monitoring during 13 Days on Satellite Mission in Outer SpaceOnline pH and DO Measurements in Microcarrier-Based hMSC CultivationsStudying Microbial Iron and Manganese Oxidation in Different Experimental Set-UpsOnline in Situ Measurements of Soil CO2 Concentrations in Dependence of Ash Roots (Fraxinus excelsior L.)Aerobic and oxygen-limited enrichment of BTEX-degrading biofilm bacteria: dominance of Malikia versus Acidovorax speciesTranscriptome-Stable Isotope Probing Provides Targeted Functional and Taxonomic Insights Into Microaerobic Pollutant-Degrading Aquifer MicrobiotaStable isotope probing for hypoxic toluene degradation at the Siklós aquifer reveals prominent role of RhodocyclaceaeIn situ measurement of oxygen consumption to estimate corrosion ratesOxic Fe(III) reduction could have generated Fe(II) in the photic zone of Precambrian seawaterTurbulence induces metabolically costly behaviors and inhibits food capture in oyster larvae, causing net energy lossComparison of test methods for oxygen permeability: Optical method versus carrier gas methodProduction of functionalized oligo-isoprenoids by enzymatic cleavage of rubberStructural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of RubberRoxB Is a Novel Type of Rubber Oxygenase That Combines Properties of Rubber Oxygenase RoxA and Latex Clearing Protein (Lcp)Effects of ascorbic acid and light on reactions in fresh-cut apples by microcalorimetryAssays for the Detection of Rubber Oxygenase ActivitiesDissolved Organic Nitrogen Inputs from Wastewater Treatment Plant Effluents Increase Responses of Planktonic Metabolic Rates to WarmingEffects of wastewater treatment plant effluent inputs on planktonic metabolic rates and microbial community composition in the Baltic SeaEffect of exposure to sunlight and phosphorus-limitation on bacterial degradation of coloured dissolved organic matter (CDOM) in freshwaterCompetition between Oxygen and Nitrate Respirations in Continous Culture of Pseudomonas aeruginosa Performing Aerobic DenitrificationEffects of Iron on Chemical Sulfide Oxidation in Wastewater from Sewer NetworksKinetics of chlorobenzene biodegradation under reduced oxygen levelsAdult neural stem cells express glucose transporters GLUT1 and GLUT3 and regulate GLUT3 expressionAerated subsurface irrigation water gives growth and yield benefits to zucchini, vegetable soybean and cotton in heavy clay soilsNew and Fast Method To Quantify Respiration Rates of Bacterial and Plankton Communities in Freshwater Ecosystems by Using Optical Oxygen Sensor SpotsOxygen consumption of a single embryo/planula in the reef-building coral Acropora intermediaBiomass-specific respiration rates of benthic meiofauna: Demonstrating a novel oxygen micro-respiration systemChlorobenzene biodegradation under consecutive aerobic-anaerobic conditionsEssential fatty acids influence metabolic rate and tolerance of hypoxia in Dover sole (Solea solea) larvae and juvenilesEvaluation of a lifetime-based optode to measure oxygen in aquatic systemsA promising environmentally-friendly manganese-based catalyst for alkyd emulsion coatingsA Novel Method to Determine Oxidation Rates of Heritage Materials in Vitro and in SituNiche specialization of reef-building corals in the mesophotic zone: metabolic trade-offs between divergent Symbiodinium typesMagnitude and regulation of bacterioplankton respiratory quotient across feshwater environmental gradientsA high-resolution non-invasive approach to quantify oxygen transport across the capillary fringe and within the underlying groundwaterAquatic Respiration Rate Measurements at Low Oxygen ConcentrationsZoogloea oleivorans sp. nov., a floc-forming, petroleum hydrocarbon-degrading bacterium isolated from biofilmVadose zone oxygen (O2) dynamics during drying and wetting cycles: An artificial recharge laboratory experimentInfluence of plasticizers on the mechanical and barrier properties of cast biopolymer filmsExperimental Sensitivity Analysis of Oxygen Transfer in the Capillary FringeConnecting bacterial colonization to physical and biochemical changes in a sand box infiltration experimentOxygen Transfer in a Fluctuating Capillary Fringe: Impact of Microbial Respiratory ActivityMetabolic rates of a hypogean and an epigean species of copepod in an alluvial aquiferComparison of an Electrochemical and Luminescence-Based Oxygen Measuring System for Use in the Biodegradability Testing According to Closed Bottle Test (OECD 301D)

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