Autoclavable Sensor for Most Precise Oxygen Measurements

Oxygen Sensor Spot SP-PSt3-YAU

Sensor spots are the most versatile version of non-invasive optical oxygen sensors. They can be attached to the inner surface of any transparent glass or plastic vessel like e. g. shake and spinner flasks, tubes, Petri dishes or cultivation bags. Oxygen is measured non-invasively and non-destructively through the transparent vessel wall. The SP-PSt3-YAU has a measurement range of 0 – 100 % oxygen in dissolved or gaseous phase. The oxygen sensitive coating is immobilized on a 1 mm glass support, which can be autoclaved (+ 130 °C, 1.5 atm).

  • Contactless measurements through the vessel wall
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measures oxygen in liquids as well as in gas phase
  • Autoclavable
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Bioprocess Development: Oxygen Monitoring in Shake Flasks

O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.

Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Specifications Gaseous & Dissolved O2 Dissolved O2
*after two-point calibration as described in the manual
Measurement range

0 – 100 % O2

0 – 1000 hPa

0 – 45 mg/L

0 – 1400 µmol/L
Limit of detection 0.03 % oxygen 15 ppb

± 0.01 % O2 at 0.21 % O2
± 0.1 % O2 at 20.9 % O2
±0.1 hPa at 2 hPa

± 1 hPa at 207 hPa

± 0.004 mg/L at 0.091 mg/L
± 0.04 mg/L at 9.1 mg/L
± 0.14 µmol/L at 2.83 µmol/L

± 1.4 µmol/L at 283.1 µmol/L
Accuracy* ± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2
Drift < 0.03 % O2 within 30 days (sampling interval of 1 min. / at 0% oxygen)
Measurement temperature range from 0 to + 50 °C
Response time (t90) < 6 sec. < 40 sec.
Compatibility Aqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivity Organic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure Steam sterilization
Ethylene oxide (EtO)
Gamma irradiation
Cleaning procedure Cleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 – 5 %
Calibration Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability 60 months provided the sensor material is stored in the dark

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Evaluation of an Optical O2 Probe and Sensor Spots for Long-Term Measurements in Stirred-Tank Bioreactors Integration of Chemical-Optical Oxygen and pH Sensors in Existing Control Units Oxygen Measurements of Innate Immune Cells in Suspension Cultures Effect of Oxygen Depletion on GDH Activity Cell Growth and Recombinant Protein Production in Small-Scale Culture Vessels Feedback Controlled Carbon Feeding at Shake Flask Scale Integration of a PreSens Oxygen Sensor Spot Into a Solid Mini Bioreactor kLa Measurement in TubeSpin Bioreactors A comprehensive comparison of mixing and mass transfer in shake flasks and their relationship with MAb productivity of CHO cells Analysis of the Proton Spin–Lattice Relaxation in Wine and Hydroalcoholic Solutions Optimization of growth and electrosynthesis of PolyHydroxyAlkanoates by the thermophilic bacterium Kyrpidia spormannii Recommendations for process engineering characterisation of single-use bioreactors and mixing systems by using experimental methods (2nd Edition) In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture Micro-Bioreactors in Space: Case Study of a Yeast (Saccharomyces cerevisiae) Bioreactor With a Non-Invasive Monitoring Method SCREENED: A Multistage Model of Thyroid Gland Function for Screening Endocrine-Disrupting Chemicals in a Biologically Sex-Specific Manner Intricate Genetic Programs Controlling Dormancy in Mycobacterium tuberculosis Microbioreactor for lower cost and faster optimisation of protein production Bioreactor-manufactured cartilage grafts repair acute and chronic osteochondral defects in large animal studies Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks High cell density cultivation of recombinant yeasts and bacteria under non-pressurized and pressurized conditions in stirred tank bioreactors Characterization and application of an optical sensor for quantification of dissolved O2 in shake flasks Fully automated single-use stirred-tank bioreactors for parallel microbial cultivations New Milliliter-Scale Stirred Tank Bioreactors for the Cultivation of Mycelium Forming Microorganisms Microfluidic Reactor for Continuous Cultivation of Saccharomyces cerevisiae Investigation of the Central Carbon Metabolism of Sorangium cellulosum: Metabolic Network Reconstruction and Quantification of Pathway Fluxes Design and development of microbioreactors for long-term cell culture in controlled oxygen microenvironments Miniature bioreactors for automated high-throughput bioprocess design (HTBD): reproducibility of parallel fed-batch cultivations with Escherichia coli Discrimination of riboflavin producing Bacillus subtilis strains based on their fed-batch process performances on a milliliter scale Reaction engineering studies for the production of 2-hydroxyisobutyric acid with recombinant Cupriavidus necator H 16 Milliliter-Scale Stirred Tank Reactors for the Cultivation of Microorganisms Growth and recombinant protein expression with Escherichia coli in different batch cultivation media Process performance of parallel bioreactors for batch cultivation of Streptomyces tendae A New Microfluidic Concept for Parallel Operated Milliliter-Scale Stirred Tank Bioreactors Reaction engineering studies of acetone-butanol-ethanol fermentation with Clostridium acetobutylicum A Liquid Ventilator Prototype for Total Liquid Ventilation Preclinical Studies A System of Miniaturized Stirred Bioreactors for Parallel Continuous Cultivation of Yeast With Online Measurement of Dissolved Oxygen and Off-Gas Delineation of the Key Aspects in the Regulation of Epithelial Monolayer Formation Shaken flasks by resonant acoustic mixing versus orbital mixing: Mass transfer coefficient kLa characterization and Escherichia coli cultures comparison A microfluidics-based in vitro model of the gastrointestinal human-microbe interface


Can I sterilize fiber optic sensors? Do I have to mount the sensors in the dark because it says "Protect from light" on the packing? Do the sensors work in turbid solutions? Does each oxygen sensor spot need a separate calibration, or are the calibration values valid for the complete sensor spot batch? How can I convert an oxygen value into a different oxygen unit? How can I prepare the calibration solutions cal0 and cal100 for oxygen sensors? How do I import data into excel from a text file containing measurement data? How do I verify whether the oxygen sensor is giving correct readings (performance proof)? How does an oxygen sensor work? How does salinity affect the oxygen measurement? How does temperature affect the oxygen measurement? How many samples should I use to perform a batch calibration? In which vessel can I integrate a sensor spot? Is the glue or the sensor spot biocompatible - which tests were done? Are there any studies on leachables and extractables? Is there a standard recommended distance to place the polymer optical fiber (POF) from the sensor spot? The Stern-Volmer-equation What are the response times for the oxygen sensors based on the 2 mm fiber like non-invasive oxygen sensors and oxygen probes? What does -YAU, -NAU or -SA stand for in the oxygen sensor spot names and what is the difference between those sensor types? What factors will affect the oxygen reading? What is the time of delivery? Where do I need sensor spot sizes different from the common diameter of 5 mm (approx. 0.2 inch)? Which glue can I use to integrate optical sensor spots? Which side of the oxygen sensor spot should face the medium? Which substances can interfere with the optical O2, pH and CO2 measurements?



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