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Autoclavable Sensor for Most Precise Oxygen Measurements

Oxygen Sensor Spot SP-PSt3-YAU

Sensor spots are the most versatile version of non-invasive optical oxygen sensors. They can be attached to the inner surface of any transparent glass or plastic vessel like e. g. shake and spinner flasks, tubes, Petri dishes or cultivation bags. Oxygen is measured non-invasively and non-destructively through the transparent vessel wall. The SP-PSt3-YAU has a measurement range of 0 – 100 % oxygen in dissolved or gaseous phase. The oxygen sensitive coating is immobilized on a 1 mm glass support, which can be autoclaved (+ 130 °C, 1.5 atm).

  • Contactless measurements through the vessel wall
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measures oxygen in liquids as well as in gas phase
  • Autoclavable

Applications

Bioprocess Development: Oxygen Monitoring in Shake Flasks

O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.


Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Technical

SpecificationsGaseous & Dissolved O2Dissolved O2
*after two-point calibration as described in the manual
Measurement range

0 – 100 % O2

0 – 1000 hPa

0 – 45 mg/L

0 – 1400 µmol/L
Limit of detection0.03 % oxygen15 ppb
Resolution

± 0.01 % O2 at 0.21 % O2
± 0.1 % O2 at 20.9 % O2
±0.1 hPa at 2 hPa

± 1 hPa at 207 hPa

± 0.004 mg/L at 0.091 mg/L
± 0.04 mg/L at 9.1 mg/L
± 0.14 µmol/L at 2.83 µmol/L

± 1.4 µmol/L at 283.1 µmol/L
Accuracy*± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2
Drift< 0.03 % O2 within 30 days (sampling interval of 1 min. / at 0% oxygen)
Measurement temperature rangefrom 0 to + 50 °C
Response time (t90)< 6 sec.< 40 sec.
Properties
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivityOrganic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedureSteam sterilization
Ethylene oxide (EtO)
Gamma irradiation
Cleaning procedureCleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 – 5 %
CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability24 months provided the sensor material is stored in the dark

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Resources

Publications

Oxygen Measurements of Innate Immune Cells in Suspension Cultures Effect of Oxygen Depletion on GDH Activity Cell Growth and Recombinant Protein Production in Small-Scale Culture Vessels Feedback Controlled Carbon Feeding at Shake Flask Scale Integration of a PreSens Oxygen Sensor Spot Into a Solid Mini Bioreactor kLa Measurement in TubeSpin Bioreactors Bioreactor-manufactured cartilage grafts repair acute and chronic osteochondral defects in large animal studies Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks High cell density cultivation of recombinant yeasts and bacteria under non-pressurized and pressurized conditions in stirred tank bioreactors Characterization and application of an optical sensor for quantification of dissolved O2 in shake flasks Fully automated single-use stirred-tank bioreactors for parallel microbial cultivations New Milliliter-Scale Stirred Tank Bioreactors for the Cultivation of Mycelium Forming Microorganisms Microfluidic Reactor for Continuous Cultivation of Saccharomyces cerevisiae Investigation of the Central Carbon Metabolism of Sorangium cellulosum: Metabolic Network Reconstruction and Quantification of Pathway Fluxes Design and development of microbioreactors for long-term cell culture in controlled oxygen microenvironments Miniature bioreactors for automated high-throughput bioprocess design (HTBD): reproducibility of parallel fed-batch cultivations with Escherichia coli Discrimination of riboflavin producing Bacillus subtilis strains based on their fed-batch process performances on a milliliter scale Reaction engineering studies for the production of 2-hydroxyisobutyric acid with recombinant Cupriavidus necator H 16 Milliliter-Scale Stirred Tank Reactors for the Cultivation of Microorganisms Growth and recombinant protein expression with Escherichia coli in different batch cultivation media Process performance of parallel bioreactors for batch cultivation of Streptomyces tendae A New Microfluidic Concept for Parallel Operated Milliliter-Scale Stirred Tank Bioreactors Reaction engineering studies of acetone-butanol-ethanol fermentation with Clostridium acetobutylicum A Liquid Ventilator Prototype for Total Liquid Ventilation Preclinical Studies A System of Miniaturized Stirred Bioreactors for Parallel Continuous Cultivation of Yeast With Online Measurement of Dissolved Oxygen and Off-Gas Delineation of the Key Aspects in the Regulation of Epithelial Monolayer Formation Shaken flasks by resonant acoustic mixing versus orbital mixing: Mass transfer coefficient kLa characterization and Escherichia coli cultures comparison A microfluidics-based in vitro model of the gastrointestinal human-microbe interface

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