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Autoclavable Sensor for Most Precise Oxygen Measurements

Oxygen Sensor Spot SP-PSt3-YAU

Sensor spots are the most versatile version of non-invasive optical oxygen sensors. They can be attached to the inner surface of any transparent glass or plastic vessel like e. g. shake and spinner flasks, tubes, Petri dishes or cultivation bags. Oxygen is measured non-invasively and non-destructively through the transparent vessel wall. The SP-PSt3-YAU has a measurement range of 0 – 100 % oxygen in dissolved or gaseous phase. The oxygen sensitive coating is immobilized on a 1 mm glass support, which can be autoclaved (+ 130 °C, 1.5 atm).

  • Contactless measurements through the vessel wall
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measures oxygen in liquids as well as in gas phase
  • Autoclavable

Applications

Bioprocess Development: Oxygen Monitoring in Shake Flasks

O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.


Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Technical

SpecificationsGaseous & Dissolved O2Dissolved O2
*after two-point calibration as described in the manual
Measurement range

0 – 100 % O2

0 – 1000 hPa

0 – 45 mg/L

0 – 1400 µmol/L
Limit of detection0.03 % oxygen15 ppb
Resolution

± 0.01 % O2 at 0.21 % O2
± 0.1 % O2 at 20.9 % O2
±0.1 hPa at 2 hPa

± 1 hPa at 207 hPa

± 0.004 mg/L at 0.091 mg/L
± 0.04 mg/L at 9.1 mg/L
± 0.14 µmol/L at 2.83 µmol/L

± 1.4 µmol/L at 283.1 µmol/L
Accuracy*± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2
Drift< 0.03 % O2 within 30 days (sampling interval of 1 min. / at 0% oxygen)
Measurement temperature rangefrom 0 to + 50 °C
Response time (t90)< 6 sec.< 40 sec.
Properties
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivityOrganic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedureSteam sterilization
Ethylene oxide (EtO)
Gamma irradiation
Cleaning procedureCleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 – 5 %
CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability24 months provided the sensor material is stored in the dark

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Resources

Publications

Oxygen Measurements of Innate Immune Cells in Suspension CulturesEffect of Oxygen Depletion on GDH ActivityCell Growth and Recombinant Protein Production in Small-Scale Culture VesselsFeedback Controlled Carbon Feeding at Shake Flask ScaleIntegration of a PreSens Oxygen Sensor Spot Into a Solid Mini BioreactorkLa Measurement in TubeSpin BioreactorsProduction of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasksHigh cell density cultivation of recombinant yeasts and bacteria under non-pressurized and pressurized conditions in stirred tank bioreactorsCharacterization and application of an optical sensor for quantification of dissolved O2 in shake flasksFully automated single-use stirred-tank bioreactors for parallel microbial cultivationsNew Milliliter-Scale Stirred Tank Bioreactors for the Cultivation of Mycelium Forming MicroorganismsMicrofluidic Reactor for Continuous Cultivation of Saccharomyces cerevisiaeInvestigation of the Central Carbon Metabolism of Sorangium cellulosum: Metabolic Network Reconstruction and Quantification of Pathway FluxesDesign and development of microbioreactors for long-term cell culture in controlled oxygen microenvironmentsMiniature bioreactors for automated high-throughput bioprocess design (HTBD): reproducibility of parallel fed-batch cultivations with Escherichia coliDiscrimination of riboflavin producing Bacillus subtilis strains based on their fed-batch process performances on a milliliter scaleReaction engineering studies for the production of 2-hydroxyisobutyric acid with recombinant Cupriavidus necator H 16Milliliter-Scale Stirred Tank Reactors for the Cultivation of MicroorganismsGrowth and recombinant protein expression with Escherichia coli in different batch cultivation mediaProcess performance of parallel bioreactors for batch cultivation of Streptomyces tendaeA New Microfluidic Concept for Parallel Operated Milliliter-Scale Stirred Tank BioreactorsReaction engineering studies of acetone-butanol-ethanol fermentation with Clostridium acetobutylicumA Liquid Ventilator Prototype for Total Liquid Ventilation Preclinical StudiesA System of Miniaturized Stirred Bioreactors for Parallel Continuous Cultivation of Yeast With Online Measurement of Dissolved Oxygen and Off-GasDelineation of the Key Aspects in the Regulation of Epithelial Monolayer FormationShaken flasks by resonant acoustic mixing versus orbital mixing: Mass transfer coefficient kLa characterization and Escherichia coli cultures comparisonA microfluidics-based in vitro model of the gastrointestinal human-microbe interface

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