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Whether it is general information or detailed, application based data on optical sensors you are looking for, in our collection of publications, sensor basics and FAQs you will find what you need. Furthermore, short tutorial videos and our instruction manuals will help you with handling your sensor system.
O2-sensitive Microcavity Arrays for 3D Cell Culture Monitoring: Oxygen Imaging in Spheroids with VisiSens TD Technology
C. Grün1, G. Liebsch2 und E. Gottwald1
1Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
Microthermoforming is applied to create an array of microcavities in oxygen sensor foils. 3D cell aggregates can be cultured in these cavities and the oxygen content can be…
Download PDFIn situ Remediation of Contaminated Groundwater: VisiSens TD used to study release of groundwater remedial amendments from well screens under flow-through conditions
Seagren E.A., Waypa A.
Department of Civil, Environmental, and Geospatial Engineering, Michigan Technological University, USA
This application note shows how the VisiSens imaging technology can be used to visualize and quantify the release of groundwater remedial amendments from unpumped wells under…
Download PDFMonitoring Spatio-Temporal Oxygen Consumption Within Live 3D Cancer Spheroids: VisiSens TD with MIC Kit Enables New Insights
Carina Schmittlein1, Robert Johannes Meier2, Christina Hupf1, Joachim Wegener1,3
1 Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany
2 PreSens Precision Sensing GmbH, Regensburg, Germany
3 Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT),
Regensburg, Germany
Testing Plant Seed Viability by Respiration Measurements: Oxygen Consumption Monitoring in 96 Well Plate with VisiSens TD and ViviPlate a Newly Developed Airtight Plate Clamp System
G. Liebsch & R. Meier
PreSens Precision Sensing GmbH, Regensburg, Germany
PreSens has developed the sensor and clamp tool ViviPlate that allows to create closed respirator chambers of different numbers and volumes in microtiterplate format. These…
Download PDFSpatio-temporal O2 Gradients in the Microenvironment of an Outgrowing Cell Patch: Oxygen Imaging in Micro-Scale with the VisiSens TD MIC System
C. Schmittlein1, R. J. Meier2, J. Wegener1,3
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT), Regensburg, Germany
Cell-based assays have emerged to an indispensable tool in all areas of the biomedical sciences. However, cell culture experiments in a laboratory environment are always questioned…
Download PDFOxygen Consumption Rates of Adherent MDCK II Monolayer Cells: A Comparison of Open and Closed Systems Using the VisiSens TD Imaging System
C. Schmittlein1, R. J. Meier2, J. Wegener1
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
Oxygen is a molecule of utmost importance for biological systems because it is necessary for life but may also induce detrimental damage. Thus, the local O2 concentration is an…
Download PDFMonitoring Oxygenation in Microfluidic Cell Culture Using 2D Sensor Foils as Growth Substrate (full text): Oxygen Imaging with VisiSens™
C. Schmittlein1, R. J. Meier2, L. Sauer1, G. Liebsch2, J. Wegener1,3
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT), Regensburg, Germany
White Paper Summary
Cells respond in very different manner regarding their microenvironment oxygenation. The oxygen availability for cells in microfluidics is strongly restricted…
Download PDFAdhesive Cell Growth and Microscopy on O2 and pH Sensor Foils: Impact of coating on the adhesion of cells upon VisiSens™ sensor foils SF-RPSu4 and SF-HP5R
C. Schmittlein1, R. J. Meier2, G. Liebsch2, J. Wegener1,3
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT), Regensburg, Germany
Suspension of Madin-Darby Canine Kidney cells (MDCK-II) were plated on VisiSens™ sensor foils SF-RPSu4 (O2) and SF-HP5R (pH) that were integrated in regular Petri dishes. Cell…
Download PDFImaging of pH and pO2 on Irradiated Fibroblasts and Oral Squamous Carcinoma Cells: Dual read-out of pH and pO2 in 96 well plates with VisiSens TD
Yvonne Reinders1, Robert J. Meier2, Gregor Liebsch2 and Frank Haubner3
1Department of Plastic, Aesthetic, Hand & Reconstructive Surgery, University Hospital Regensburg, Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Department of Otorhinolaryngology, Ludwig-Maximilians-University Munich, Munich, Germany
Oxygen Dynamics around Buried Tar Balls in Florida Marine Sands: A laboratory flume study testing the VisiSensTM oxygen imaging system
Markus Huettel
Department of Earth, Ocean and Atmospheric Science, Florida State University, FL, USA
After the Deepwater Horizon accident in 2011, crude oil in unknown amounts was embedded in sandy shelf and shore sediments of the northeastern Gulf of Mexico. Oxygen is a key…
Download PDFPerfusion Monitoring in Microvascular Flaps: Fluorescence Imaging Applied as a Method to Discover Vessel Thrombosis
J. K. Meier1, M. Gosau1, S. Müller1, A. Moralis1, G. Liebsch2, and L. Prantl3
1Department of Cranio-Maxillo-Facial Surgery, University Medical Center Regensburg, Germany; 2PreSens GmbH, Regensburg, Germany; 3Center of Plastic-, Hand-, and Reconstructive Surgery, University Medical Center Regensburg, Germany
Tissue transfer in head and neck reconstruction has a high success rate. Nevertheless, in up to 10 % of all cases serious complications occur. Skin flap monitoring with technical…
Download PDFMeasuring Respiration and Primary Production Dynamics in Macroalgae: 2D imaging of oxygen gradients in F. vesiculosus with the VisiSens system
K. Spilling
Finnish Environment Institute, Marine Research Laboratory, Helsinki, Finland
In this study the VisiSens system was used for 2D imaging of small scale differences in primary production and respiration in macroalgae. F. vesiculosus was used as a model…
Download PDFViability of Lithobiontic Microorganisms Inhabiting the Rocks of Atacama Desert: Determining photosynthetic activity with VisiSens
Jacek Wierzchos and Carmen Ascaso
Museo Nacional de Ciencias Naturales - CSIC, Madrid, Spain
The hyper-arid, life-limited Atacama Desert (Chile) gives the opportunity to determine the potential for life in extraterrestrial environments. In this study the viability of…
Download PDFOxygen Dynamics in the Capillary Fringe: Mapping oxygen consumption of Pseudomonas fluorescens with VisiSens
Norman Hack & Harald Horn
Chair of Water Chemistry and Water Technology, Engler-Bunte-Institut, Karlsruhe Institute of Technology, Germany
The capillary fringe is a highly active zone for chemical and biological transformation at the transition of the vadose zone and groundwater. To study aerobic biodegradation in the…
Download PDFMonitoring Skin Tissue Oxygenation in Mice: Comparing VisiSens with other Oxygen Measurement Methods
Julian Hofmann1, Robert M. Meier2, Alexander Mahnke1, Valentin Schatz1, Gregor Liebsch3, Otto Wolfbeis2, and Jonathan Jantsch1
1Mikrobiologisches Institut - Institute of Clinical Microbiology, Immunology and Hygiene, University Hospital Erlangen, Germany
2Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Germany
3PreSens GmbH, Regensburg, Germany
There is a great interest in getting more information on the role of tissue oxygenation in various diseases. In this study three different measurement techniques including the…
Download PDFRecording Spatial Patterns of Oxygen Consumption in Individual Corals: Assessing Coral Health Under a Changing Climate with VisiSens
Hollie M. Putnam, Denise M. Yost, Ruth D. Gates
Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, USA
The calcified skeleton of corals makes it difficult to take biological measurements. The VisiSens imaging system was applied to measure oxygen consumption patterns in individual…
Download PDFEffect of Non-Symbiotic Hemoglobin Expression on Oxygen Content in Roots: Oxygen Imaging and Respiration Measurement in Roots with VisiSens
Jagadis Gupta Kapuganti
Biochemistry & Systems Biology, Department of Plant Sciences, University of Oxford, GB
This project aimed to define the role of plant non-symbiotic hemoglobin and regulation of respiration by nitric oxide in plant roots. Wild-type and hemoglobin over-expressing…
Download PDFEffect of Pseudomonas Infection on Oxygen Status of Arabidopsis Leaves: Visualizing Oxygen Dynamics in Plant Tissue
Jagadis Gupta Kapuganti
Biochemistry & Systems Biology, Department of Plant Sciences, University of Oxford, GB
In order to prevent pathogens from spreading, infected plants use a form of cell death - the so called hypersensitive response. In this study the widely used model system of Arabid…
Download PDFMonitoring Oxygen in a Mouse Model of Renovascular Hypertension: Checking Microvascular Perfusion in Mouse Kidney with the VisiSens System
Zoltán Varga
Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Hungary
In this study we used a mouse model of renovascular hypertension known as the two-kidney, one clip model (2K1C). In this model one renal artery is constricted which ultimately…
Download PDFBenthic Disturbance-Recovery Dynamics in a Changing Coastal Ocean: Imaging the effects of terrestrial sediment deposits on intertidal sandflat pH and O2 with VisiSens technology
Kay Vopel, Aysha Hohaia, Chris Pook, and John Robertson
Institute for Applied Ecology New Zealand, Auckland University of Technology, New Zealand
Changes in climate and land use increase the supply of terrestrial sediment to coastal water worldwide but the effects of this increase on ecosystem functioning are not well known.…
Download PDFExchange of O2 and CO2 in the Capillary Fringe of a Porous Medium: A laboratory study testing the VisiSens imaging systems
Christina Haberer1, Massimo Rolle1,2, and Peter Grathwohl1
1Department of Geosciences, University of Tübingen, Germany
2Department of Civil and Environmental Engineering, Stanford University, USA
Diffusion experiments were performed in a quartz sand packing to study the effect of evaporative conditions on mass transfer of oxygen and CO2 across the interface between the…
Download PDFRole of Oak Wood Ellagitannins on the Oxygen Transfer Rate in Wine Barrels: 2D Visualization of Oxygen Permeation with VisiSens
I. Nevares Domínguez, M. del Alamo, C. González, and R. Crespo
UVaMOX, Universidad de Valladolid, Palencia, Spain
Oxygen permeation through oak wood in a wine barrel scenario has been recorded with the VisiSens™ oxygen imaging system. A special measurement chamber was designed to simulate…
Download PDFImaging the Oxygen Consumption of Microbial Cultures: Metabolic activity of E. coli monitored inside the incubator with VisiSens A1
H. Tschiersch1, G. Liebsch2, L. Borisjuk1, A. Stangelmayer2, H. Rolletschek1
1Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
Oxygen imaging with VisiSens™ A1 allows 2-dimensional mapping of oxygen consumption inside microbial culture. The compact measurement system can be applied for monitoring the…
Download PDFMonitoring pO2 in Cell Culture to Improve Directed Differentiation: Visualizing oxygen distributions in 6 well plates with VisiSens A1
S. M. Rolandin1,2, C. Fraker1, V. Manzoli1, C. Villa1,2, A. A. Tomei1, Y. Torrente2, L. Inverardi1, J. Dominguez-Bendala1
1Diabetes Research Institute, University of Miami Miller School of Medicine, USA
2Stem Cell Laboratory, Department of Pathophysiology and Transplantation, Fondazione Ca´Granda IRCCS, Ospedale Maggiore Policlinico, Centro Dino Ferrari, Universita di Milano, IT
Tissue oxygenation during in vitro culture of stem cells is a critical factor. Previous studies have shown that maintaining pO2 during in vitro culture at levels approximating the…
Download PDFImaging Rhizosphere pH and CO2 Dynamics: Plant Root - Soil Interactions Quantified with Prototype VisiSens Systems
S. Blossfeld1, C. M. Schreiber2, G. Liebsch3, A. J. Kuhn1, and P. Hinsinger2
1Forschungszentrum Jülich GmbH, Institute of Bio- and Geosciences, IBG-2: Plant sciences, Jülich, Germany
2INRA, UMR Eco&Sol, Montpellier, France
3PreSens Precision Sensing GmbH, Regensburg, Germany
Prototype VisiSens™ devices for pH & CO2 imaging were used to perform continuous and highly resolved measurements in rhizobox systems. pH was measured around durum wheat roots…
Download PDFExtracorporeal Membrane Oxygenator to Support Diseased Lungs: Monitoring the gas exchange capacity of hollow fibers with VisiSens
Michael Pflaum
HTTG / LEBAO - Hanover Medical School, Germany
The function of a diseased lung can be supported by the application of an extracorporeal membrane oxygenator (ECMO). Aim of this study is to improve the hemocompatibility of the…
Download PDFAssessment of Oxygen Depletion and Biofilm Structure Grown in MBBR Carriers: Tracking the Distribution of Oxygen Penetration through Biofilm Carriers with VisiSens
E. Gilbert, S. Lackner, M. Wagner, and H. Horn
Chair of Water Chemistry and Water Technology, Engler-Bunte-Institut, Karlsruhe Institute of Technology, Germany
Biofilm systems like moving bed biofilm reactors (MBBR) are used for wastewater treatment. Preliminary tests were conducted to evaluate the suitability of 2D oxygen imaging for…
Download PDFMeasuring Oxygen Gradients above a Respiratory Active Algal Cell Layer: VisiSens Applied to Detect Dynamic Oxygen Fluctuations over Time
Cindy Ast, Carola Päpke, and Joost T. van Dongen
Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany
Oxygen gradients in buffer solution above a monolayer of Chlamydomonas reinhardtii have been visualized with the VisiSens system. Aim of the experiment was to determine, if…
Download PDFImmobilized Plant Cells - From DSMZ to the Customer: VisiSens technology used to detect metabolic activities of immobilized plant cells during simulated cell transport in a closed system
Nicole Brinkmann, Martina Westphal, Elke Heine-Dobbernack, and Martin Schumacher
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany
The DSMZ is one of the largest biological resource centers worldwide. Its collections currently comprise almost 40,000 items, including about 700 undifferentiated plant cell lines.…
Download PDFDiffusive / Dispersive Reaction Fronts in Groundwater: 2D visualization of oxygen and pH using the VisiSens imaging system to study Fe2+ oxidation under flow-through conditions
Christina Haberer1, Muhammad Muniruzzaman1, Massimo Rolle1,2, and Peter Grathwohl1
1Department of Geosciences, University of Tübingen, Germany
2Department of Civil and Environmental Engineering, Stanford University, USA
Quasi 2D flow-through experiments were performed in heterogeneous porous media to study the oxidation of ferrous iron in groundwater using the VisiSens™ imaging system for oxygen…
Download PDFBiogenic Gas Bubbles Produced within Soil Macropores: Simultaneous measurement of gas bubble size and CO2 concentration with VisiSens A3
Yuhong He, Cameron Proctor, and Alex Tong
Department of Geography and Planning, University of Toronto, Canada
Greenhouse gas measurements in wetlands note considerable spatial and temporal variability in biogenic gas bubbles, suggesting microscale heterogeneities influence formation and…
Download PDFIntegration of Oxygen Sensor Foils in Microfluidic Chips: Applicability of VisiSens in Micro-Channels made by Ultrasonic Welding
L. Burs, A. Witte, J. Wlodarczyk, T. Nieradzik, W. K. Schomburg
Faculty of Mechanical Engineering, KEmikro, RWTH-Aachen University, Germany
We evaluated the VisiSens™ A1 imaging system for its applicability in micro-channels to create a microfluidic chip with integrated oxygen sensing. The experiments described here…
Download PDFCellular Oxygen Consumption in Microfluidic Devices: Monitoring oxygen tensions with the VisiSens system
Christopher Ochs1, Junichi Kasuya2 and Andrea Pavesi1
1Singapore MIT Alliance for Research and Technology, BioSystems and Micromechanics, Singapore
2MechanoBiology Laboratory, Department of Biological Engineering, Massachusetts Institute of Technology, MA, US
Microfluidic devices fabricated from cyclic olefin copolymer (COC) and polydimethylsiloxane (PDMS) were combined with VisiSens sensor foils to enable monitoring of oxygen tensions…
Download PDFAssessing Biocide Actions on Lichen with VisiSens: Controlling microbial biodeterioration on a dolostone Roman bridge
Carmen Ascaso and Jacek Wierzchos
Museo Nacional de Ciencias Naturales - CSIC, Madrid, Spain
Historical monuments are often subject to microbial bio deterioration. Effective treatments that inhibit biological growth on ancient rock surfaces have to be found. In this study…
Download PDFO2, CO2 and pH Dynamics in the Capillary Fringe: VisiSens combined with an automated linear positioning system provides complete analyte maps
M. Wagner, N. Hack, G. Abbt-Braun, and H. Horn
Chair of Water Chemistry and Water Technology, Engler-Bunte-Institut, Karlsruhe Institute of Technology, Germany
We investigated the biochemical transformation potential in the capillary fringe (CF) by measuring the distribution of dissolved gases and the pH value. Therefore, we combined the…
Download PDFOptical pH Surveillance in a Microfluidic System for Cell Culture: Evaluation of VisiSens pH sensor foils integrated in microfluidic systems
A. Schopf1,2, C. Boehler1,2, M Asplund1,2
1Laboratory for Biomedical Microtechnology, Department of Microsystems Engineering (IMTEK), University of Freiburg, Germany
2Brain Tools Cluster of Excellence, University of Freiburg, Germany
pH surveillance within microfluidic systems is a challenging problem. Liquid quantities available for measurement are limited and also relatively inaccessible within the…
Download PDFA New Non-invasive Technique for Measuring 3D-Oxygen Gradients in Wells During Mammalian Cell Culture
Carlos J. Peniche Silva, Gregor Liebsch, Robert J. Meier, Martin S. Gutbrod, Elizabeth R. Balmayor and Martijn van Griensven
Front. Bioeng. Biotechnol. (2020) 8:595
Elevated micro-topography boosts growth rates in Salicornia procumbens by amplifying a tidally-driven oxygen pump: implications for natural recruitment and restoration
Gregory S. Fivash, Jim van Belzen, Ralph J. M. Temmink, Karin Didderen, Wouter Lengkeek, Tjisse van der Heide, Tjeerd J. Bouma
Annals of Botany (2019)
Tomato plants rather than fertilizers drive microbial community structure in horticultural growing media
Oliver Grunert, Ana A. Robles-Aguilar, Emma Hernandez-Sanabria, Silvia D. Schrey, Dirk Reheul, Marie-Christine Van Labeke, Siegfried E. Vlaeminck, Tom G. L. Vandekerckhove, Mohamed Mysara, Pieter Monsieurs, Vicky M. Temperton, Nico Boon & Nicolai D. Jablonowski
Scientific Reports (2019) 9:9561
A microfluidic oxygen sink to create a targeted cellular hypoxic microenvironment under ambient atmospheric conditions
Samineh Barmaki, Ville Jokinen, Daniela Obermaier, Daria Blokhina, Matti Korhonen, Robin H.A. Ras, Jyrki Vuola, Sami Franssila, Esko Kankuri
Acta Biomaterialia (2018)
Online Monitoring of Crude Oil Biodegradation at Elevated Pressures
Ana Gabriela Valladares Juárez, Hari Spandana Kadimesetty, Daniela E. Achatz, Martina Schedler, and Rudolf Müller
IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing (2015) Vol. 8, No. 2, 872 - 878
Low-oxygen tensions found in Salmonella-infected gut tissue boost Salmonella replication in macrophages by impairing antimicrobial activity and augmenting Salmonella virulence
Jonas Jennewein, Jasmin Matuszak, Steffi Walter, Boas Felmy, Kathrin Gendera, Valentin Schatz, Monika Nowottny, Gregor Liebsch, Michael Hensel, Wolf-Dietrich Hardt, Roman G. Gerlach, and Jonathan Jantsch
Cellular Microbiology (2015)
Disentangling who is who during rhizosphere acidification in root interactions: combining fluorescence with optode techniques
Marc Faget, Stephan Blossfeld, Philipp von Gillhaussen, Ulrich Schurr, and Vicky M. Temperton
Frontiers in Plant Science, Functional Plant Ecology, Vol. 4, Article 392, 2013
Ratiometric luminescence 2D in vivo imaging and monitoring of mouse skin oxygenation
Julian Hofmann, Robert J Meier, Alexander Mahnke, Valentin Schatz, Florian Brackmann, Regina Trollmann, Christian Bogdan, Gregor Liebsch, Xu-dong Wang, Otto S Wolfbeis, and Jonathan Jantsch
Methods and Applications in Fluorescence 1 (2013)
Characterization of a chip-based bioreactor for three-dimensional cell cultivation via magnetic resonance imaging
Eric Gottwald, Tanja Kleintschek, Stefan Giselbrecht, Roman Truckenmüller, Brigitte Altmann, Matthias Worgull, Jörg Döpfert, Lothar Schad, Melanie Heilmann
Z. Med. Phys., 2013
Transcutaneous pO2 imaging during tourniquet-induced forearm ischemia using planar optical oxygen sensors
Philipp Babilas, Philipp Lamby, Lukas Prantl, Stephan Schreml, Ernst Michael Jung, Gregor Liebsch, Otto S. Wolfbeis, Michael Landthaler, Rolf-Markus Szeimies and Christoph Abels
Skin Research and Technology 14, 304-311, 2008