Watch tutorials, webinars and informative videos about PreSens optical sensor systems.
Find Publications and Information about Your Optical Sensor System
Whether it is general information or detailed, application based data on optical sensors you are looking for, in our collection of publications, sensor basics and FAQs you will find what you need. Furthermore, short tutorial videos and our instruction manuals will help you with handling your sensor system.
Online Monitoring of C. glutamicum Culture in Ultra Yield® Flasks: SFR vario Used with Only Slightly Translucent Shake Flasks
C. Ude and G. T. John
PreSens Precision Sensing GmbH, Regensburg, Germany
In this study we analyzed, if slightly translucent polypropylene shake flasks can be used for optical measurements and especially optical biomass monitoring with the SFR vario. As…
Download PDFWhy It Is Important to Measure Different Parameters Online in Shake Flasks: SFR vario Measures O2, OUR, pH and Biomass Online
R. W. Maschke1, G. T. John2, and D. Eibl1
1 ZHAW Zurich University of Applied Sciences, School of Life Sciences and Facility Management, Wädenswil, Switzerland
2PreSens Precision Sensing GmbH, Regensburg, Germany
Optical sensors enable monitoring and controlling important culture parameters even at shake flask scale. In this short report we show an example of multiparameter monitoring with…
Download PDFCharacterizing CHO Cell Lines with SFR vario: What online measured data can tell about your cell culture
R. W. Maschke1, B. Pretzner2, G. T. John3, D. Eibl1
1Zurich University of Applied Sciences, Institute of Chemistry and Biotechnology, Wädenswil, Switzerland
2Werum IT Solutions GmbH, Vienna, Austria
3PreSens Precision Sensing GmbH, Regensburg, Germany
Imaging of pH and pO2 on Irradiated Fibroblasts and Oral Squamous Carcinoma Cells: Dual read-out of pH and pO2 in 96 well plates with VisiSens TD
Yvonne Reinders1, Robert J. Meier2, Gregor Liebsch2 and Frank Haubner3
1Department of Plastic, Aesthetic, Hand & Reconstructive Surgery, University Hospital Regensburg, Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Department of Otorhinolaryngology, Ludwig-Maximilians-University Munich, Munich, Germany
Improving Culture Conditions of Antibody-Producing Yeast: Use of Deep Well SensorDishes® for pH and DO Monitoring
Lotta Kirjavainen and Alexander Frey
Aalto University, Finland
Reliable screening set-ups are the key to rapid identification of genetic modifications or changes of cultivation conditions improving strain performance. Moreover, online…
Download PDFLight vs. Dark Oxygen Demand of Plankton Samples: O2 Measurements with SensorVials and the SDR SensorDish® Reader
Dr Sabine Schultes
Aquatic Ecology Group, LMU Munich, Germany
The dynamics of production and consumption of O2 in natural plankton communities change along environmental gradients and yield important information on the community ecology and…
Download PDFQuality Assessment of Primary Human Keratinocyte Cultures: Online Measurement of Oxygen and pH with the SDR SensorDish® Reader
V. Lorenz, B. Roski, A. Hermann, and H. F. Abts
Celonic GmbH, Jülich, Germany
The SDR SensorDish® Reader allows non-invasive analysis of dissolved oxygen (DO) and pH in multiwell plates. In this sutdy the sensitivity of the monitoring device was tested in…
Download PDFOptical Biomass Measurements in a Salvia fruticosa Cell Culture: Evaluation of the SFR vario for plant cell applications
P. Scherhag1, G. T. John2, C. Haas3 and J.-U. Ackermann1
1University of Applied Sciences, Department of Biochemistry and Bioengineering, Dresden, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Technical University, Institute fo Natural Materials Technology, Dresden, Germany
Suspension culture of plant cells is gaining importance in production of pharmaceutically active substances as it is independent from environmental influences. In order to…
Download PDFThe Application of the SFR Shake Flask Reader in Teaching at the Esslingen University of Applied Sciences: Non-invasive Measurements of Dissolved Oxygen and pH in Shake Flasks within the Framework of Practical Courses and Student Projects
A. Komitakis, K. Schatz, M. Hoß, R. Biener
Department Biotechnology, Esslingen University of Applied Sciences, Germany
Dissolved oxygen (DO) and pH are key parameters for microbial fermentations and cell cultures. The importance of DO and pH for bioprocesses as well as methods for their measurement…
Download PDFCO2, Oxygen and Biomass Monitoring in E. coli Shake Flask Culture: Following Glucose-Glycerin Diauxie Online with the SFR vario
Helga Tietgens1, Gernot T. John2 and Katja Bettenbrock1
1Max Planck Institut für Dynamik komplexer technischer Systeme, Magdeburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
Diauxic growth of E. coli in medium containing glycerin and glucose was monitored online with the SFR vario. The device measures biomass online via scattered light detection.…
Download PDFAccurate Insight into Oxygen Content of Shake Cultures
Michael Findeis and Gernot T. John
PreSens GmbH, 93053 Regensburg, Germany
Numerous factors affect growth in cell culture in shake flasks. The free oxygen content plays a decisive role. Insufficient oxygen input from the environment limits growth. With…
Download PDFDevelopment of a Partly Controllable System at Shake Flask Scale: Enhanced data quality obtained from fed-batch shake flask cultivation by minimizing disturbance variables monitored by the SFR
Christian Klinger, Sylvia Baumann, Robert Puskeiler, and Alexander Jockwer
Roche Diagnostics GmbH, Pharma Biotech Production and Development, Penzberg, Germany
The main objective of this study was to develop an easy to handle and partly controllable process cultivation system at shake flask scale. First, it was investigated how…
Download PDFInvestigation on ATP Synthesis via Oxidative Phosphorylation in C. glutamicum: Measurement of oxygen consumption by an ATP synthase deletion mutant and the wild type strain with the SFR Shake Flask Reader
A. Koch-Koerfges1, A. Kabus1, I. Ochrombel2, K. Marin2, and M. Bott1
1Institute for Bio- and Geosciences (IBG-1), Forschungszentrum Jülich, Germany
2Institue for Biochemistry, University of Cologne, Germany
A F1F0-ATP synthase deletion mutant of C. glutamicum was characterized and investigated in respect to growth, by-product formation and bioenergetics. The mutant did not grow with…
Download PDFOnline Monitoring of Glucose, pH, and DO in Shake Flask Culture: Novel Glucose Sensor Used in Combination with the SFR Shake Flask Reader
I. Bauer1, G. T. John2, S. Spichiger3, and U. E. Spichiger-Keller3
1Zurich University of Applied Sciences, School of Life Sciences and Facility Management, Institute of Biotechnology, Biochemical Engineering and Cell Cultivation Technique, Waedenswil, Switzerland
2PreSens Precision Sensing GmbH, Regensburg, Germany
3C-CIT AG, Waedenswil, Switzerland
A new single-use glucose sensor in combination with chemical optical oxygen and pH sensors was used for online monitoring of CHO cell culture in shake flasks. During the whole…
Download PDFkLa Measurement in TubeSpin Bioreactors
T. Backoff1, J. Malig1, S. Werner2, G. T. John3, D. Eibl2
1Anhalt University of Applied Sciences, Department 7 - Applied Biosciences and Process Engineering, Köthen, Germany
2Zurich University of Applied Sciences, School of Life Sciences and Facility Management, Institute of Biotechnology, Wädenswil, Switzerland
3PreSens Precision Sensing GmbH, Regensburg, Germany
TubeSpin bioreactors are used for cell cultivation as a cost effective alternative to larger scale cultivation systems. However, the characteristics of the tubes with regard to…
Download PDFDevelopment of a Shaken Scale-Down Model: Characterization of Critical Parameters in Bioprocess Development
Holger Kley, Jan Kreuzmann, Caroline Schuster, and Robert Puskeiler
Roche Diagnostics GmbH, Pharma Biotech Production and Development, Penzberg, Germany
A shaken scale-down model at approx. 0.1 L scale for a previously established stirred-tank model at 2 L scale was developed. In the shaken scale-down model online monitoring of pH…
Download PDFCharacterization of Microbial Growth Profiles: Online detection of oxygen and pH during culture in shake flasks
Konstantin Schneider1, Verena Schütz1, Gernot Thomas John2 and Elmar Heinzle1
1Biochemical Engineering Institute, Saarland University, 66123 Saarbrücken, Germany
2PreSens Precision Sensing GmbH, 93053 Regensburg, Germany
Shake flasks are the most commonly used cultivation systems for process and media optimization, screening for new products and strains as well as for the supply of inoculum for…
Download PDFInsect Cell Cultivation in Orbitally Shaken Flasks with Sensors: Oxygen mass transfer in insect cell-based high cell density cultivations
N. Riesen, C. Ries, R. Eibl, D. Eibl
Zurich University of Applied Sciences, School of Life Sciences and Facility Managment, Institute of Biotechnology, Biochemical Engineering and Cell Cultivation Techniques, Wädenswil, Switzerland
Insect cell-based processes have become increasingly influential for tool protein and Virus-like Particle (VLP) vaccine productions over the last years. At small scale insect cells…
Download PDFA New Method for Predicting Oxygen Supply during Aerobic Growth: Measurements with the SFR Shake Flask Reader allow kLa determination and reliable design of culture conditions in disposable shake flasks
S. Schiefelbein1, A. Fröhlich1, G. T. John2, F. Beutler2, C. Wittmann1, and J. Becker1
1Institute of Biochemical Engineering, Technische Universität Braunschweig, Germany
2PreSens Precision Sensing GmbH, Germany
Dissolved oxygen (DO) is a key parameter during aerobic cultivation and due to its low solubility oxygen can become limiting under certain culture conditions. In this study a new…
Download PDFNew Device for Biomass Monitoring in Shake Flask Culture: Development of an analytical unit based on the SFR Shake Flask Reader
J. Schmidt-Hager1, C. Ude1, M. Findeis2, G. T. John2, T. Scheper1, and S. Beutel1
1Institute of Technical Chemistry, Leibniz University of Hanover, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
We developed a novel system for online biomass measurement in shake flasks based on near 360° scattered light detection. The optosensoric components were combined with the…
Download PDFOnline Monitoring of S. cerevisiae Culture with the SFR vario: New online biomass data is in very good accordance with offline measurements
Dylan Haskew1, Gernot T. John2, and Dogan Ornek1
1Upstream Process Development, FUJIFILM Diosynth Biotechnologies U. S. A., Inc., US
2PreSens Precision Sensing GmbH, Germany
In order to investigate a model process with Saccharomyces cerevisiae the new SFR vario shake flask reader was used to monitor O2, OUR, pH, and biomass online inside 2 L shake…
Download PDFOnline Oxygen Monitoring System: Non-conventional steroid fermentations in microtiter plates
Marques M.P.C., Cabral J.M.S., Fernandes P.
Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Lisboa, Portugal
A key factor when optimising and developing bioprocesses is the monitoring of the fermentation, in order to quantify in real-time basis parameters like oxygen, pH and carbon…
Download PDFNon-invasive Method for Monitoring Real-Time Oxygen during Hematopoietic Stem/Progenitor Cell (HSPC) Culture
Timothy McKinnon1, Nelli Baal2, and Marek Zygmunt2
1Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
2Universitätsklinikum Gießen und Marburg, Germany
The SDR SensorDish® Reader from PreSens has allowed for the quantification of in vitro oxygen concentrations during the culture of hematopoietic stem/progenitor cells (HSPC)…
Download PDFA Novel Online Monitoring System: For Non-Invasive Oxygen Detection in Chondrocyte Cultures
Bettina Giere
Tierärztliche Hochschule Hannover, Germany
For optimisation of cell culture systems it is essential to monitor important metabolic parameters like oxygen and pH quantitatively and to readjust them to physiological values if…
Download PDFOxygen and pH Monitoring in Deep Well OxoDishes® and HydroDishes®: Assessing optimal growth conditions for shaken yeast culture
Thomas Grimm
BIOWORX, Berlin, Germany
In this study different yeast cultivations in deep well OxoDishes® and HydroDishes® were examined. Cultivations were carried out at varying volumes to assess optimal growth…
Download PDFLocalized Oxygen Depletion by Transmigrating Neutrophils Influences Inflammatory Resolution: Oxygen monitoring in cell-cell cross talk experiments with the SDR SensorDish® Reader
Eric L. Campbell, and Sean Colgan
Mucosal Inflammation Program, University of Colorado, Anschutz Medical Campus, USA
Recent research on intestinal inflammation has shown that mucosal metabolism influences disease outcome. Intestinal inflammation is accompanied by accumulation of neutrophils…
Download PDFCorrecting for Oxygen Diffusion in Respiration Measurements: Determining instantaneous oxygen consumption rate in OxoDishes® applied as closed-system respirometers
E. T. Polymeropoulos1,2, N. G. Elliott2, S. J. Wotherspoon3, P. B. Frappell1,3
1School of Zoology, University of Tasmania, AU
2Commonwealth Scientific and Industrial Research Organisation (CSIRO), National Food Futures Flagship, CSIRO Marine and Atmospheric Research, AU
3Institute for Marine and Antarctic Studies, University of Tasmania, AU
When using multiwell plates made of polysytrene - like the PreSens OxoDishes® - as closed-system respirometers, one has to take oxygen diffusion through the well material into…
Download PDFReal Time Respiration Measurements: Measuring the aerobic respiration of complex microbial communities
B. R. Roller1, Z. M. Lee1, K. E. Studer-Rabeler2, and T. M. Schmidt1
1Dept. of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA
2Coy Laboratory Products, Grass Lake, MI, USA
Many methods for measuring aerobic respiration, including electrodes and colorimetric assays, limit the capacity for obtaining replicate measurements or the ability to detect…
Download PDFHigh Throughput Respiration Assay for Cytotoxicity Determination: A Method for the Evaluation of New Active Pharmaceutical Ingredients Using the SDR SensorDish® Reader
Marie-Theres Weil, and Tobias Werner
Institute of Analytical Chemistry, University of Applied Sciences Mannheim, Germany
The objective of this project was to develop a cytotoxicity assay with high throughput by monitoring the cellular respiration in cell culture. Cells were cultured in 24-well plates…
Download PDFCo-Cultures of Caco-2 cells and E.coli: Optosensoric online monitoring of O2 uptake and extracellular acidification
Martin Brischwein and Daniel Grundl
Heinz-Nixdorf-Lehrstuhl für Medizinische Elektronik, TU München
Oligogalacturonic acids (OGAs), obtained by hydrolysis of pectin, are known to inhibit the adhesion of pathogenic bacteria on intestinal cells and thus to prevent infectious…
Download PDFOnline Oxygen Monitoring in Cell Culture: Effects of mitochondrial modulators on O2 dynamics of mammalian cells
Lynn S. G. and LaPres J. J.
Dept. of Biochemistry, Michigan State University, East Lansing, MI, USA
Real-time measurements of oxygen and pH in mammalian cell cultures are important to understand the metabolic dynamics in cell cultures. The SDR SensorDish® Reader allows the time…
Download PDFNon-Invasive Online Oxygen Measurement: Bacterial toxicity tests for use in pharmacological agent
Anna Vasilevskaya
Institute for Analytical Chemistry, University of Regensburg, Germany
Measurement of oxygen kinetics in respiration inhibition tests is of central importance for pharmacological agent research and toxicology testing of chemicals (REACH). The new SDR…
Download PDFProcess Monitoring in Suspension-Adapted CHO Cell Cultures: Non-invasive online detection of pH and oxygen
Harry Abts1 and Sarina Arain2
1Celonic GmbH, Karl-Heinz-Beckurts-Straße 13, Jülich, Germany
2PreSens GmbH, Josef-Engert-Str. 11, 93053 Regensburg, Germany
Online detection of oxygen and pH by using SDR at a small-scale can be used as a basis for latter cultivation in large-scale bioreactors to detect optimal growth conditions.…
Download PDFEnergy Catabolism Measurements in Metastatic Macrophage M3 Cells: Cell respiration measurements with the SDR SensorDish Reader and the XF 96 Extracellular Flux Analyzer
Robert Flores1, Brian Coy2, Karen Studer-Rabeler2, and Thomas N. Seyfried1
1Biology Department, Boston College, Chestnut Hill, MA, USA
2Coy Laboratory Products, Inc., Grass Lake, MI, USA
In order to study the role of mycoplasma infection on cancer cell energy catabolism, we measured oxygen consumption and lactate production in either infected or non-infected…
Download PDFDesign of Experiment (DoE) for Fed-batch Process Development in Shaken Cultures: Determination of reproducibility and scalability in Escherichia coli cultures grown under fed-batch conditions using SensorDishes® and Sensor Flasks
F. Glauche, N. Cruz, A. Knepper, M. Föllmer, S. Junne, P. Neubauer
Laboratory of Bioprocess Engineering, Department of Biotechnology, TU Berlin, Germany
When designing a recombinant protein production process, a high number of parallel cultivations need to be carried out. This is mostly performed using batch cultures in shake…
Download PDF3D Cultures of Disc-Chondrocytes: Optosensoric Online Monitoring of Oxygen and pH Kinetics
Karin Benz and Jürgen Mollenhauer
Regenerative Medizin II, NMI Reutlingen, Markwiesenstraße 55, 72770 Reutlingen, Germany
Hydrogel-based 3D cell culture systems offer particular advantages for tissue-engineering in cell-based therapy for disc-degeneration. However, light microscopical control of both…
Download PDFEvaluating the SDR SensorDish® Reader for Strain Development: Monitoring different E. coli strains inside deep well OxoDishes®
Olaf Christensen
Lonza Ltd., Visp, Switzerland
We conducted several tests to evaluate the applicability of the SDR SensorDish® Reader combined with deep well OxoDishes® in strain development. We measured oxygen consumption of…
Download PDFIdentifying Water Oxidation Catalysts for Clean Fuel Production: High throughput screening of water oxidation reactions with OxoDishes®
R. J. Detz1, Z. Abiri1,2, A. M. Kluwer2, J. N. H. Reek1
1Van't Hoff Institute for Molecular Sciences, University of Amsterdam, NL
2InCatT B. V., Amsterdam, NL
The splitting of water in a light-driven reaction is one of the most promising processes for producing clean fuel. In order to create an efficient process suitable reaction…
Download PDFValidation Study of the Optrode Dual™: Oxygen and pH Measurements in a Single-Use Bioreactor with Integrated Optical Sensors Using a Conventional Controller
Stephan Kaiser & Dieter Eibl
Institute for Biotechnology, Department of Life Sciences and Facility Management, Zurich University of Applied Sciences, Switzerland
The Optrode Dual™ converts measurement data of optical sensors into electrochemical signals (ECS) which can be transferred to controller units for bioreactors. The user can record…
Download PDFOptrode Dual Connects Single-Use Bioreactors to Conventional Controllers: Monitoring of CHO Cell Cultivation with Chemical Optical Sensors
Stephan Kaiser, Franziska Fietz, Nina Steiger, and Dieter Eibl
Institute for Biotechnology, Department of Life Sciences and Facility Management, Zurich University of Applied Sciences, Switzerland
The Optrode Dual™ prototype has been applied for measurements in suspension adapted CHO cell culture. The transmitter converts the reading of chemical optical sensors integrated in…
Download PDFCell Growth and Recombinant Protein Production in Small-Scale Culture Vessels: Optical oxygen sensor spots help optimizing culture conditions
V. Jäger1, G. Apati1,2, and N. Konisch1
1Recombinant Gen Expression Group, Helmholtz Centre for Infection Research, Braunschweig, Germany
2Universidade da Região de Joinville, Brazil
Optical measurement of oxygen allows the introduction of small sensor spots into the culture vessels. As many of the conventional sub-bioreactor scale culture vessels are not…
Download PDFContinuous optical in-line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality
Mario Lederle, Mircea Tric, Tatjana Roth, Lina Schütte, Anke Rattenholl, Dirk Lütkemeyer, Stefan Wölfl, Tobias Werner, Philipp Wiedemann
Biotechnol. J. (2021) 16:2100088
Recommendations for process engineering characterisation of single-use bioreactors and mixing systems by using experimental methods (2nd Edition)
I. Bauer, T. Dreher, D. Eibl, R. Glöckler, U. Husemann, G. T. John, S. C. Kaiser, P. Kampeis, J. Kauling, S. Kleebank, M. Kraume, W. Kuhlmann, C. Löffelholz, W. Meusel, J. Möller, R. Pörtner, C. Sieblist, B. Tscheschke, S. Werner
DECHEMA Publications (2020)
HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of Online Monitoring in Shake Flasks (RAMOS and SFR)
Tibor Anderlei, Michael V. Keebler, Jordi Joan Cairó, and Martí Lecina
In: Pörtner R. (eds) Animal Cell Biotechnology. Methods in Molecular Biology, vol 2095. Humana, New York, NY (2020) 83 - 103
Application of an Online-Biomass Sensor in an Optical Multisensory Platform Prototype for Growth Monitoring of Biotechnical Relevant Microorganisms and Cell Lines in Single-Use Shake Flasks
Christian Ude, Jörg Schmidt-Hager, Michael Findeis, Gernot T. John, Thomas Scheper, Sascha Beutel
Sensors, 2014, 14, 17390 - 17405
Pioglitazone modulates tumor cell metabolism and proliferation in multicellular tumor spheroids
Eva Gottfried, Sebastian Rogenhofer, Heidi Waibel, Leoni A. Kunz-Schughart, Albrecht Reichle, Monika Wehrstein, Alice Peuker, Katrin Peter, Gabi Hartmannsgruber, Reinhard Andreesen, Marina Kreutz
Cancer Chemother Pharmacol (2010)